R-Spondin 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling R-Spondin 1 with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 15 ug of spleen lysates per lane. Antibodies: RSPO1,5171 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL), 1h incubationat RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgGHRP conjugate at 1:10000 dilution.

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Analysis in human heart tissue lysate with antibody at (A) 1 and (B) 2 ug/mL.

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of lysates per lane. Lane 1: 0.5ug/ml, Lane 2: 1ug/ml

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of human RSPO1 recombinant protein perlane. Antibodies: RSPO1, 5171 (Lane 1: 0.5 ug/mL, Lane 2:1 ug/mL, Lane 3: 2 ug/mL), 1h incubation at RT in 5%NFDM/TBST. Secondary: Goat anti-rabbit IgG HRPconjugate at 1:10000 dilution.

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Heart. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-R-Spondin 1 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-R-Spondin 1 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-R-Spondin 1 antibody at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

R-Spondin 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit R-Spondin 1 Antibody - BSA Free (NBP1-77354) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-R-Spondin 1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human R-Spondin 1. The immunogen is located within the first 50 amino acids of R-Spondin 1. Amino Acid Sequence: RGIKGKRQRRISAEGS
• Specificity: At least three isoforms of R-Spondin 1 are known to exist; this antibody will detect the largest and smallest isoforms. R-Spondin 1 antibody will not cross-react with other RSPO family members. Human R-Spondin 1 has 3 isoforms, including isoform 1 (263aa, 29kD), isoform 2 (236aa, 26kD), and isoform 3 (200aa, 22kD). Mouse R-Spondin 1 had 1 isoform (265aa, 29kD) and rat R-Spondin 1 had 1 isoform (262aa, 29kD). NBP1-77354 can detect human, mouse and rat.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RSPO1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2.5-5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/mL
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  R-Spondin 1 Peptide (NBP1-77354PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for R-Spondin 1 Antibody - BSA Free

• Cristin 3
• CRISTIN3
• FLJ40906Roof plate-specific spondin-1
• HRspo1
• roof plate-specific spondin
• RSPO
• RSPO1
• RSpondin 1
• R-Spondin 1
• R-spondin homolog (Xenopus laevis)
• RSPONDIN
• R-spondin1
• R-spondin-1

Background

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for R-Spondin 1 Antibody (NBP1-77354)(3)

Publications using NBP1-77354

• Shen Y, Wang J, Dai Y et al. RSPO3 Promotes Proliferation and Self-Renewal of Limbal Epithelial Stem Cells Through a WNT/ beta -Catenin-Independent Signaling Pathway Investigative Ophthalmology & Visual Science 2025-01-06 [PMID: 39760688]
• MY Turco, L Gardner, RG Kay, RS Hamilton, M Prater, MS Hollinshea, A McWhinnie, L Esposito, R Fernando, H Skelton, F Reimann, FM Gribble, A Sharkey, SGE Marsh, S O'Rahilly, M Hemberger, GJ Burton, A Moffett Trophoblast organoids as a model for maternal-fetal interactions during human placentation Nature, 2018-11-28;0(0):. 2018-11-28 [PMID: 30487605]
• Takahashi Y, Sato S, Kurashima Y et al. A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids. Stem Cell Reports 2017-12-01 [PMID: 29233552] (Human)

Bioinformatics

• Gene Symbol: RSPO1
• Uniprot:
  • NP_001033722(Human)