R-Spondin 1 Antibody

Images

 
Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Analysis in human heart tissue lysate with antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody [NBP1-77354] - Staining of RSPO1 in human spleen tissue with RSPO1 antibody at 5 ug/ml.
Immunohistochemistry: R-Spondin 1 Antibody [NBP1-77354] - RSPO1 in human spleen tissue with RSPO1 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody [NBP1-77354] - Human Spleen cells.
Immunohistochemistry-Paraffin: R-Spondin 1 Antibody [NBP1-77354] - Human spleen tissue with RSPO1 antibody at 2.5 ug/ml.
Immunohistochemistry: R-Spondin 1 Antibody [NBP1-77354] - Analysis of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

R-Spondin 1 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.
Specificity
At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RSPO1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
R-Spondin 1 Peptide (NBP1-77354PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for R-Spondin 1 Antibody

  • Cristin 3
  • CRISTIN3
  • FLJ40906Roof plate-specific spondin-1
  • hRspo1
  • roof plate-specific spondin
  • RSPO
  • RSPO1
  • RSpondin 1
  • R-Spondin 1
  • R-spondin homolog (Xenopus laevis)
  • RSPONDIN
  • R-spondin1
  • R-spondin-1

Background

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RSPO1
Entrez
Uniprot