R-Spondin 1 Proteins

1324-WN)." class="big_lightbox"> 1324-WN)." alt="Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (1324-WN)." class="big_thumb" />

1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red)." class="big_lightbox"> 1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red)." alt="Recombinant Human R-Spondin 1 (Catalog # 4645-RS), in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red)." class="big_thumb" />

1324-WN). Each trace on the graph represents data obtained from Recombinant Human R-Spondin 1 from a different manufacturing run. The ED₅₀ for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a." class="big_lightbox"> 1324-WN). Each trace on the graph represents data obtained from Recombinant Human R-Spondin 1 from a different manufacturing run. The ED₅₀ for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a." class="big_thumb" />

236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (5748; blue)." class="big_lightbox"> 236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (5748; blue)." class="big_thumb" />

236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; red) and DAPI (5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; red) and DAPI (5748; blue). The image shown was taken at 20x magnification." class="big_lightbox"> 236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; red) and DAPI (5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; red) and DAPI (5748; blue). The image shown was taken at 20x magnification." class="big_thumb" />

1324-WN)." class="big_lightbox"> 1324-WN)." alt="Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 50-200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN)." class="big_thumb" />

4645-RS) induces activation of beta -catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional." class="big_lightbox"> 4645-RS) induces activation of beta -catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional." alt="Both Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645) and unlabeled Recombinant Human R-Spondin 1 (Catalog # 4645-RS) induces activation of beta -catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional." class="big_thumb" />