R-Spondin 1 Antibodies
We offer R-Spondin 1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Western Blot. Each R-Spondin 1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our R-Spondin 1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the R-Spondin 1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our R-Spondin 1 polyclonal antibodies and browse our R-Spondin 1 monoclonal antibody catalog.
Alternate Names for R-Spondin 1 Antibodies
anti-R-Spondin 1 antibody, anti-RSPO1 antibody, anti-CRISTIN3 antibody, anti-FLJ40906Roof plate-specific spondin-1 antibody, anti-hRspo1 antibody, anti-R-spondin homolog (Xenopus laevis) antibody, anti-R-spondin-1 antibody, anti-R-spondin1 antibody, anti-roof plate-specific spondin antibody, anti-RSPO antibody, anti-RSPONDIN antibody
27 Results for "r-spondin-1" in Primary Antibodies
NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="R-Spondin 1 was detected in immersion fixed frozen sections of E13.5 mouse ovary using Goat Anti-Mouse R-Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3474) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />
7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R-Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R-Spondin 1 Mono-clonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (1324-WN). The ND50 is typically 0.4-2 µg/mL." alt="Recombinant Mouse R-Spondin 1 (7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R-Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R-Spondin 1 Mono-clonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (1324-WN). The ND50 is typically 0.4-2 µg/mL." class="big_thumb" />
![Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling R-Spondin 1 with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/antibody/nbp1-77354_rabbit-polyclonal-r-spondin-1-antibody-immunocytochemistry-immunofluorescence-18122024192515..gif "Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling R-Spondin 1 with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).")
![Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 15 ug of spleen lysates per lane. Antibodies: RSPO1,5171 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL), 1h incubationat RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgGHRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-Western-Blot-NBP1-77354-img0014.jpg)
![Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Analysis in human heart tissue lysate with antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-Western-Blot-NBP1-77354-img0008.jpg)
![Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of lysates per lane. Lane 1: 0.5ug/ml, Lane 2: 1ug/ml](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-Western-Blot-NBP1-77354-img0012.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of human RSPO1 recombinant protein perlane. Antibodies: RSPO1, 5171 (Lane 1: 0.5 ug/mL, Lane 2:1 ug/mL, Lane 3: 2 ug/mL), 1h incubation at RT in 5%NFDM/TBST. Secondary: Goat anti-rabbit IgG HRPconjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-Western-Blot-NBP1-77354-img0013.jpg)
![Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Heart. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-R-Spondin 1 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77354_rabbit-polyclonal-r-spondin-1-antibody-271120242017171.gif)
![Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-R-Spondin 1 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77354_rabbit-polyclonal-r-spondin-1-antibody-271120241928834.gif)
![Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-R-Spondin 1 antibody at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77354_rabbit-polyclonal-r-spondin-1-antibody-2711202419451231.jpg)
![Western Blot: R-Spondin 1 Antibody (11A9) [NBP2-45636] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY R-Spondin 1.](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-11A9-Western-Blot-NBP2-45636-img0002.jpg "Western Blot: R-Spondin 1 Antibody (11A9) [NBP2-45636] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY R-Spondin 1.")
![Immunohistochemistry: R-Spondin 1 Antibody (11A9) [NBP2-45636] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-11A9-Immunohistochemistry-NBP2-45636-img0001.jpg)
![Immunohistochemistry-Paraffin: R-Spondin 1 Antibody [NBP1-92357] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/R-Spondin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92357-img0006.jpg "Immunohistochemistry-Paraffin: R-Spondin 1 Antibody [NBP1-92357] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.")
