QPRT Antibody (PSH01-31)

Western Blot: QPRT Antibody (PSH01-31) [NBP3-32881] - Western blot analysis of QPRT on different lysates with Rabbit anti-QPRT antibody (NBP3-32881) at 1/1,000 dilution. Lane 1: HepG2 cell lysate, 20 ug/Lane Lane 2: MCF7 cell lysate, 20 ug/Lane Lane 3: SK-Br-3 cell lysate, 20 ug/Lane Lane 4: HEK-293 cell lysate, 20 ug/Lane Lane 5: HeLa cell lysate, 20 ug/Lane Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32881) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: QPRT Antibody (PSH01-31) [NBP3-32881] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-QPRT antibody (NBP3-32881) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32881) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PSH01-31
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

QPRT Antibody (PSH01-31) Summary

• Description: Novus Biologicals Rabbit QPRT Antibody (PSH01-31) (NBP3-32881) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human QPRT aa 1-297 / 297. (Uniprot: Q15274)
• Localization: Catalytic complex, cytoplasm, cytosol, extracellular exosome.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: QPRT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for QPRT Antibody (PSH01-31)

• EC 2.4.2.19
• nicotinate-nucleotide pyrophosphorylase (carboxylating)
• QAPRTase
• QPRTasenicotinate-nucleotide pyrophosphorylase [carboxylating]
• Quinolinate phosphoribosyltransferase [decarboxylating]
• quinolinate phosphoribosyltransferase

Background

QPRT encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: QPRT