QPRT Antibody (OTI1C10) - Azide and BSA Free

Western Blot: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY QPRT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-QPRT.

Immunocytochemistry/Immunofluorescence: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY QPRT.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human lymphoma tissue using anti-QPRT mouse monoclonal antibody.

Flow Cytometry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-QPRT antibody, and then analyzed by flow cytometry.

Western Blot: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Analysis of extracts (35ug) from 9 different cell lines by using anti-QPRT monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-QPRT mouse monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-QPRT mouse monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-QPRT mouse monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-QPRT mouse monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human endometrium tissue using anti-QPRT mouse monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human liver tissue using anti-QPRT mouse monoclonal antibody.

Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human prostate tissue using anti-QPRT mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

QPRT Antibody (OTI1C10) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human QPRT (NP_055113) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: QPRT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 30.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for QPRT Antibody (OTI1C10) - Azide and BSA Free

• EC 2.4.2.19
• nicotinate-nucleotide pyrophosphorylase (carboxylating)
• QAPRTase
• QPRTasenicotinate-nucleotide pyrophosphorylase [carboxylating]
• Quinolinate phosphoribosyltransferase [decarboxylating]
• quinolinate phosphoribosyltransferase

Background

QPRT encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: QPRT