PUMA Antibody [FITC]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Order Details

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Catalog# & Conjugate Size Price

PUMA Antibody [FITC] Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human PUMA (within residues 1-100). [NCBI Sequence NP_001120712]
Localization
Mitochondrial
Specificity
This is specific for isoform 1 of PUMA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BBC3
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This PUMA antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~24 kDa. In ICC/IF mitochondrial staining was observed.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PUMA Antibody [FITC]

  • BCL2 binding component 3
  • bcl-2-binding component 3
  • JFY1
  • JFY-1
  • p53 up-regulated modulator of apoptosis
  • PUMAFLJ42994

Background

PUMA (p53 upregulated modulator of apoptosis) belongs to pro-apoptotic members of Bcl2 family's BH3-only subgroup, and besides its most critical role as mediator of p53/TP53-dependent/-independent apoptosis, it acts as a regulator of ER stress-induced neuronal apoptosis also. PUMA is expressed ubiquitously and predominately localizes to mitochondria where it execute induction of cytochrome c release. PUMA mediates apoptosis via direct binding with anti-apoptotic members of the Bcl-2 family and is capable of interacting with MCL1, BCL2A1, BCL2 and BCL2L1/BCL-XL. PUMA binding to inhibitory members of Bcl-2 family (Bcl-2-like proteins) leads to Bax and/or Bak displacement mediated activation and formation of pore-like structures over mitochondrial membrane, which permeabilizes the outer mitochondrial membrane, resulting in mitochondrial dysfunction and caspase activation. PUMA is induced by p53, DNA damage, glucocorticoid treatment, deprivation of growth factors etc. and through ER stress in a DDIT3/CHOP-dependent manner. PUMA has been reported to involve in multiple physiological and pathological processes, including immune response, cancer, neurodegenerative diseases and bacterial as well as viral infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PUMA Antibody (NB110-81760F) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PUMA Antibody (NB110-81760F)

Discover related pathways, diseases and genes to PUMA Antibody (NB110-81760F). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PUMA Antibody (NB110-81760F)

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Pathways for PUMA Antibody (NB110-81760F)

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Research Areas for PUMA Antibody (NB110-81760F)

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Blogs on PUMA.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Bioinformatics

Gene Symbol BBC3