PUM1 Antibody (JG37-75)

Western Blot: PUM1 Antibody (JG37-75) [NBP2-75638] - Analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: PUM1 Antibody (JG37-75) [NBP2-75638] - Staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PUM1 Antibody (JG37-75) [NBP2-75638] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: PUM1 Antibody (JG37-75) [NBP2-75638] - Analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PUM1 Antibody (JG37-75) [NBP2-75638] - Staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PUM1 Antibody (JG37-75) [NBP2-75638] - Analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PUM1 Antibody (JG37-75) [NBP2-75638] - Analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PUM1 Antibody (JG37-75) [NBP2-75638] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

Product Discontinued

PUM1 Antibody (JG37-75) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PUM1 aa 79-223 . (SwissProt: Q14671 Human; SwissProt: Q80U78 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PUM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:1000-1:2000
• Theoretical MW: 126 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PUM1 Antibody (JG37-75)

• HSPUM
• KIAA0099PUML1
• PUM1
• PUMH
• PUMH1
• PUMH1pumilio homolog 1
• pumilio (Drosophila) homolog 1
• pumilio homolog 1 (Drosophila)
• Pumilio-1
• PUML1

Background

Pumilio-1 encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PUM1