PUF60 Antibody

Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906] - FFPE section of human prostate carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-PUF60 used at a dilution of 1:200 (1ug/ml)Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Pm-Or
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PUF60 Antibody Summary

• Description: Novus Biologicals Rabbit PUF60 Antibody (NBP1-49906) is a polyclonal antibody validated for use in IHC and WB. Anti-PUF60 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 509 and 559 of human Poly-U Binding Splicing Factor 60KDa using the numbering given in entry NP_510965.1 (GeneID 22827).
• Predicted Species: Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PUF60
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PUF60 Antibody

• FBP interacting repressor
• FBP-interacting repressor
• FIRpoly-U binding splicing factor PUF60
• FLJ31379
• FUSE-binding protein-interacting repressor
• poly(U)-binding-splicing factor PUF60
• poly-U binding splicing factor 60KDa
• Ro ribonucleoprotein binding protein 1
• Ro ribonucleoprotein-binding protein 1
• Ro-binding protein 1
• roBP1
• RoBPI
• siah binding protein 1,60 kDa poly(U)-binding-splicing factor
• Siah-binding protein 1
• siah-BP1
• SIAHBP1pyrimidine tract binding splicing factor

Background

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PUF60 Antibody (NBP1-49906)(1)

Publication using NBP1-49906

• Yang G, Xiang J, Yang X et al. Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2 eLife 2024-11-04 [PMID: 39495216]

Bioinformatics

• Gene Symbol: PUF60