FOLR1 Antibody (548908) [Unconjugated]

Images

 
Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed ...read more
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (MAB002, open histogram), followed by ...read more
FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the ...read more
Genetic Strategies: FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (MAB002, open ...read more
(A) Immunofluorescent (left) and flow cytometry (right) detection of the EpCAM expression in MCF7 cells and the FR alpha expression in A2780 cells. EpCAM and FR alpha stained with Alexa Fluor® 488 are green at an ...read more
HeLa cells were stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap), ICC/IF, KO
Clone
548908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

FOLR1 Antibody (548908) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328
Specificity
Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOLR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL
Application Notes
ELISA Capture: Human FOLR1 Antibody (Catalog # MAB5646)
ELISA Detection: Human FOLR1 Biotinylated Antibody (Catalog # BAF5646)
Standard: Recombinant Human FOLR1 (Catalog # 5646-FR)
Reviewed Applications
Read 2 Reviews rated 5
using
MAB5646 in the following applications:

Publications
Read Publications using
MAB5646 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR1 Antibody (548908) [Unconjugated]

  • Adult folate-binding protein
  • FBP
  • folate binding protein
  • folate receptor 1 (adult)
  • Folate receptor 1
  • folate receptor alpha
  • Folate receptor, adult
  • Folbp1
  • FOLR
  • FOLR1
  • FR-alpha
  • KB cells FBP
  • MOv18
  • Ovarian tumor-associated antigen MOv18

Background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).
  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOLR1 Antibody (MAB5646)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Capture), Flow Cytometry, Western Blot.


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ELISA Capture
(3)
ELISA Detection
(1)
ELISA Development
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB5646 Applications Species
Spicer, J;Basu, B;Montes, A;Banerji, U;Kristeleit, R;Miller, R;Veal, GJ;Corrigan, CJ;Till, SJ;Figini, M;Canevari, S;Barton, C;Jones, P;Mellor, S;Carroll, S;Selkirk, C;Nintos, G;Kwatra, V;Funingana, IG;Doherty, G;Gould, HJ;Pellizzari, G;Nakamura, M;Ilieva, KM;Khiabany, A;Stavraka, C;Chauhan, J;Gillett, C;Pinder, S;Bax, HJ;Josephs, DH;Karagiannis, SN; Safety and anti-tumour activity of the IgE antibody MOv18 in patients with advanced solid tumours expressing folate receptor-alpha: a phase I trial Nature communications 2023-07-25 [PMID: 37491373] (ELISA Capture, Human) ELISA Capture Human
Yokoi, A;Ukai, M;Yasui, T;Inokuma, Y;Hyeon-Deuk, K;Matsuzaki, J;Yoshida, K;Kitagawa, M;Chattrairat, K;Iida, M;Shimada, T;Manabe, Y;Chang, IY;Asano-Inami, E;Koya, Y;Nawa, A;Nakamura, K;Kiyono, T;Kato, T;Hirakawa, A;Yoshioka, Y;Ochiya, T;Hasegawa, T;Baba, Y;Yamamoto, Y;Kajiyama, H; Identifying high-grade serous ovarian carcinoma-specific extracellular vesicles by polyketone-coated nanowires Science advances 2023-07-07 [PMID: 37418532] (Western Blot, Human) Western Blot Human
HJ Bax, J Chauhan, C Stavraka, A Santaolall, G Osborn, A Khiabany, M Grandits, J López-Aben, LCGF Palhares, C Chan Wah H, A Robinson, A Pope, N Woodman, C Naceur-Lom, S Malas, JEM Coumbe, M Nakamura, R Laddach, S Mele, S Crescioli, AM Black, S Lombardi, S Canevari, M Figini, A Sayasneh, S Tsoka, K FitzGerald, C Gillett, S Pinder, M Van Hemelr, R Kristeleit, S Ghosh, A Montes, J Spicer, SN Karagianni, DH Josephs Folate receptor alpha in ovarian cancer tissue and patient serum is associated with disease burden and treatment outcomes British Journal of Cancer, 2022-11-19;0(0):. 2022-11-19 [PMID: 36402875] (ELISA Capture, Human) ELISA Capture Human
J Song, LJ Sokoll, DW Chan, Z Zhang Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays Biomedicines, 2021-12-14;9(12):. 2021-12-14 [PMID: 34944713] (ELISA Capture, ELISA Detection, Human) ELISA Capture, ELISA Detection Human
K Taniuchi, T Yawata, M Tsuboi, T Ueba, T Saibara Efficient delivery of small interfering RNAs targeting particular mRNAs into pancreatic cancer cells inhibits invasiveness and metastasis of pancreatic tumors Oncotarget, 2019-04-23;10(30):2869-2886. 2019-04-23 [PMID: 31080558] (Western Blot, Human) Western Blot Human
Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR? Sci Rep, 2016-08-31;6(0):32427. 2016-08-31 [PMID: 27576898] (Flow Cytometry, Human) Flow Cytometry Human
Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women. PLoS ONE, 2014-05-08;9(5):e96542. 2014-05-08 [PMID: 24810481] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity. Clin. Exp. Allergy, 2011-05-16;41(10):1400-1413. 2011-05-16 [PMID: 21569129] (ELISA Development, Human) ELISA Development Human

Reviews for FOLR1 Antibody (MAB5646) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB5646:
Filter by Applications
Flow
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry FOLR1 MAB5646
Enlarge
5
reviewed by:
Verified Customer
Flow Human 04/07/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedIGROV cell line,IGROV1 cell line
SpeciesHuman
LotCCQX0817111
Flow Cytometry FOLR1 MAB5646
Enlarge
5
reviewed by:
Verified Customer
Flow Human 03/21/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman cancer cell line
SpeciesHuman
LotCCQX0817111

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Recent Reviews

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Verified Customer
04/07/2019
Application: Flow
Species: Human

Verified Customer
03/21/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol FOLR1
Uniprot