FOLR1 Antibody (548908) [Unconjugated]

Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.

MCF‑7 human breast cancer cell line was stained with Mouse Anti‑Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ELISA(Cap), ICC/IF, KO
• Clone: 548908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FOLR1 Antibody (548908) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
  Arg25-Met233
  Accession # P15328
• Specificity: Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL
• Application Notes: ELISA Capture: Human FOLR1 Antibody (Catalog # MAB5646)
  ELISA Detection: Human FOLR1 Biotinylated Antibody (Catalog # BAF5646)
  Standard: Recombinant Human FOLR1 (Catalog # 5646-FR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR1 Antibody (548908) [Unconjugated]

• Adult folate-binding protein
• FBP
• folate binding protein
• folate receptor 1 (adult)
• Folate receptor 1
• folate receptor alpha
• Folate receptor, adult
• Folbp1
• FOLR
• FOLR1
• FR-alpha
• KB cells FBP
• MOv18
• Ovarian tumor-associated antigen MOv18

Background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
7. Ross, J.F. et al. (1994) Cancer 73:2432.
8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOLR1 Antibody (MAB5646)(5)

Publications using MAB5646

• J Song, LJ Sokoll, DW Chan, Z Zhang Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays Biomedicines, 2021;9(12):. 2021 [PMID: 34944713] (ELISA Capture, Human)
• K Taniuchi, T Yawata, M Tsuboi, T Ueba, T Saibara Efficient delivery of small interfering RNAs targeting particular mRNAs into pancreatic cancer cells inhibits invasiveness and metastasis of pancreatic tumors Oncotarget, 2019;10(30):2869-2886. 2019 [PMID: 31080558] (Western Blot, Human)
• Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR? Sci Rep, 2016;6(0):32427. 2016 [PMID: 27576898] (Flow Cytometry, Human)
• Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women. PLoS ONE, 2014;9(5):e96542. 2014 [PMID: 24810481] (ELISA Development (Capture), Human)
• Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity. Clin. Exp. Allergy, 2011;41(10):1400-1413. 2011 [PMID: 21569129] (ELISA Development, Human)

Reviews for FOLR1 Antibody (MAB5646) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Ben Marcow Flow Human 04/07/2019

Summary

• Application: Flow Cytometry
• Sample Tested: IGROV cell line,IGROV1 cell line
• Species: Human
• Lot: CCQX0817111

reviewed by: Serena Adam Flow Human 03/21/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Human cancer cell line
• Species: Human
• Lot: CCQX0817111

Bioinformatics

• Gene Symbol: FOLR1
• Uniprot:
  • P15328(Human)