PTRF Antibody

Western Blot: PTRF Antibody [NB100-60635] - Detection of Human and Mouse PTRF on HeLa whole cell lysate using NB100-60635. PTRF was also immunoprecipitated by rabbit anti-PTRF antibodies NB100-60633 and NB100-60634.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PTRF Antibody Summary

• Description: Novus Biologicals Rabbit PTRF Antibody (NB100-60635) is a polyclonal antibody validated for use in WB and IP. Anti-PTRF Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 238 and 288 human RNA polymerase I and transcript release factor using the numbering given in entry NP_036364.2 (GeneID 284119).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAVIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTRF Antibody

• CAVIN
• cavin-1
• CGL4
• FLJ90031
• polymerase I and transcript release factor
• RNA polymerase I and transcript release factor
• TTF-I interacting peptide 12

Background

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTRF Antibody (NB100-60635)(2)

Publications using NB100-60635

• Vogel ER, Manlove LJ, Kuipers I et al. Caveolin-1 Scaffolding Domain Peptide Prevents Hyperoxia-Induced Airway Remodeling in a Neonatal Mouse Model Am. J. Physiol. Lung Cell Mol. Physiol. 2019-05-01 [PMID: 31042080]
• Sathish V, Thompson MA, Sinha S et al. Inflammation, caveolae and CD38-mediated calcium regulation in human airway smooth muscle. Biochim Biophys Acta. 2013-11-22 [PMID: 24275509] (WB, Human)

Bioinformatics

• Gene Symbol: CAVIN1
• Entrez:
  • 284119(Human)
  • 19285(Mouse)
• Uniprot:
  • Q6NZI2(Human)