PTRF Antibody - BSA Free

Immunoprecipitation: PTRF Antibody [NB100-60634] - Detection of Human PTRF by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTRF antibody NB100-60634 used for IP at 3 mcg/mg lysate. PTRF was also immunoprecipitated by rabbit anti-PTRF antibodies NB100-60633 and NB100-60635, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PTRF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PTRF Antibody - BSA Free (NB100-60634) is a polyclonal antibody validated for use in IP. Anti-PTRF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human RNA polymerase I and transcript release factor using the numbering given in entry NP_036364.2 (GeneID 284119).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAVIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTRF Antibody - BSA Free

• CAVIN
• cavin-1
• CGL4
• FLJ90031
• polymerase I and transcript release factor
• RNA polymerase I and transcript release factor
• TTF-I interacting peptide 12

Background

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTRF Antibody (NB100-60634)(1)

Publication using NB100-60634

• Davalos A, Fernandez-Hernando C, Sowa G et al. Quantitative proteomics of caveolin-1-regulated proteins: characterization of polymerase i and transcript release factor/CAVIN-1 IN endothelial cells. Mol Cell Proteomics 2010-10-01 [PMID: 20585024]

Bioinformatics

• Gene Symbol: CAVIN1
• Entrez:
  • 284119(Human)
• Uniprot:
  • NP_036364.2(Human)