PTPRCAP Antibody - BSA Free

Western Blot: PTPRCAP Antibody [NBP2-36565] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit PTPRCAP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: PTPRCAP Antibody [NBP2-36565] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. For blotting immunoprecipitated PTPRCAP, it was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PTPRCAP Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 156 to 206 of human Lymphocyte Phosphatase-Associated Phosphoprotein using the numbering given in entry NP_005599.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPRCAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTPRCAP Antibody - BSA Free

• CD45-APCD45 associated protein
• CD45-associated protein
• LPAPMGC138603
• Lymphocyte phosphatase-associated phosphoprotein
• MGC138602
• protein tyrosine phosphatase receptor type C-associated protein
• protein tyrosine phosphatase, receptor type, c polypeptide-associated protein
• protein tyrosine phosphatase, receptor type, C-associated protein
• PTPRC-associated protein

Background

PTPRCAP is encoded by this gene was identified as a transmembrane phosphoprotein specifically associated with tyrosine phosphatase PTPRC/CD45, a key regulator of T- and B-lymphocyte activation. The interaction with PTPRC may be required for the stable expression of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRCAP
• Uniprot:
  • NP_005599.1(Human)