PTPN3 [p Ser459] Antibody - Azide Free

Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Hu, Mu, Bv, Ca, Av-Bi, Pm, Sh, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

PTPN3 [p Ser459] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 conjugated to KLH. Accession # P26045
• Modification: p Ser459
• Specificity: Specific for endogenous levels of the ~104 kDa PTPN3 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Bovine (100%), Finch (100%), Zebrafish (100%), Xenopus (100%), Primate (100%), Sheep (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for PTPN3 [p Ser459] Antibody - Azide Free

• DKFZp686N0569
• EC 3.1.3.48
• protein tyrosin phosphatase H1
• protein tyrosine phosphatase, non-receptor type 3
• Protein-tyrosine phosphatase H1
• PTPH1
• PTP-H1
• PTPH1cytoskeletal-associated protein tyrosine phosphatase
• PTPN3
• tyrosine phosphatase
• tyrosine-protein phosphatase non-receptor type 3

Background

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38gamma MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38 gamma and PTPH1 protein expression, and there is a coupling of increased p38gamma and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN3
• Entrez:
  • 5774(Human)
• Uniprot:
  • P26045(Human)