PTPN13/PTPL1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56140] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:1000 conc. of PTPN13 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PTPN13 was observed. Staining was performed by Histowiz.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PTPN13/PTPL1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 1760-1775 (EPTRQENWTPLKNDLE) of human FAP-1 was used as immunogen; GenBank no. NP_542414.1. This peptide sequence is conserved in multiple isoforms of FAP-1. These FAP-1 isoforms are of varying amino acid (aa) lengths and include: (1) NP_542414.1 = 2485 aa, FAP-1 isoform 1. (2) BAD92141.1 = 2434 aa, FAP-1 isoform 2. (3) NP_542415.1 = 2294 aa, FAP-1 isoform 3. (4) NP_542416.1 = 2490 aa, FAP-1 isoform 4. (5) AAH39610.1 = 604 aa, FAP-1. (6) AAF63474.1 = 499 aa, FAP-1. For additional information on FAP-1 isoforms, users are encouraged to use the NCBI data bases such as AceView (http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/) or BLAST (http://www.ncbi.nlm.nih.gov/BLAST/).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN13
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Application Notes: FAP-1 typically migrates at ~270 kDa on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights. The actual molecular weight of FAP-1 observed depends on the isoform expression.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for PTPN13/PTPL1 Antibody - BSA Free

• EAP-1
• FAP-1
• hPTPE1
• PNP1
• protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associatedphosphatase)
• protein-tyrosine phosphatase 1, Fas-associated
• PTP1E
• PTP1EDKFZp686J1497
• PTP-BAS
• PTP-BASEC 3.1.3.48
• PTP-BL
• PTP-E1
• PTPL1
• PTPL1nonreceptor type 13
• PTPLE
• PTPN13
• tyrosine-protein phosphatase non-receptor type 13

Background

FAP-1 (also known as Fas-associated phosphatase-1, PTPN13, PTP-BAS, hPTPIE, and PTPL1) is a member of the protein tyrosine phosphatase (PTP) family (reviewed in Meinhold-Heerlein et al, 2001; Savaskan et al, 2005; Foehr et al, 2005; and Ivanov et al, 2006). PTPs are enzymes that catalyze the removal of a phosphate group attached to a tyrosine residue. Most intracellular signaling involves reversible phosphorylation events; therefore, PTPs are central to the dynamic regulation of signaling cascades that underlie cell functions. For example, PTPs play key roles in regulating cell growth, differentiation, proliferation, inflammation, and oncogenic transformation. PTPs are emerging as a promising class of signaling targets for diseases such as cancer, neurodegeneration, diabetes, and inflammation. A key challenge is to identify specific PTPs that are invovled in a disease process and develop therapeutics to modulate the PTP. FAP-1 phosphatase is thought to be important in the Fas signaling pathway. FAP-1 binds to the cytosolic tail of the Fas receptor (Apo1, CD95) and inhibits Fas-induced apoptosis. Increased FAP-1 protein levels in some tumor cell lines and tumor tissues correlates with resistance to Fas-mediated apoptosis. In general, FAP-1 expression has been found to be highest in cell lines and tissues that are relatively resistant to Fas-mediated apoptosis. Gene transfer-mediated elevations in FAP-1 partially abolished Fas-induced apoptosis in a T cell line which is consistent with an inhibitory effect of FAP-1 on Fas signal transductions. Additionally, FAP-1 expression correlates with Fas resistant in ovarian cancer cell lines and FAP-1 is commonly expression in ovarian cancers. Human Fas has a putative consensus tyrosine phosphorylation site (Tyrosine 275) suggesting that Fas surface expression or signaling may be regulated by phosphorylation. FAP-1 has been shown to directly bind to Fas and may dephosphorylate Fas as part of the down regulation of the apoptotic pathway. It is thought that development of therapeutics to inhibit FAP-1 may increase the ability of tumor cells with upregulated FAP-1 to undergo apoptosis. FAP-1 is a large approx. 270 kDa protein. Multiple alternatively spliced FAP-1 transcript variants which encode distinct proteins have been reported, including shorter forms. Please see Antigen section for additional details on FAP-1 isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN13
• Entrez:
  • 5783(Human)
• Uniprot:
  • Q12923()