PTP1B/PTPN1 Antibody [Unconjugated]

PTP1B was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the perinuclear region in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 57 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

PTP1B/PTPN1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PTP1B
• Specificity: Detects endogenous human PTP1B in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP1B/PTPN1 Antibody [Unconjugated]

• EC 3.1.3.48
• protein tyrosine phosphatase 1B
• protein tyrosine phosphatase, non-receptor type 1
• Protein-tyrosine phosphatase 1B
• PTP1B
• PTP-1B
• PTP1Bprotein tyrosine phosphatase, placental
• PTPN1
• tyrosine-protein phosphatase non-receptor type 1

Background

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
5. Elchebly, et al. (1999) Science 283:1544.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP1B/PTPN1 Antibody (AF1366)(8)

Publications using AF1366

• Dong Young Kim, Jeong Ae Park, Yeomyung Kim, Minyoung Noh, Songyi Park, Eunkyung Lie et al. SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175 The FASEB Journal 2019-09-01 [PMID: 31170000]
• S Torii, C Kubota, N Saito, A Kawano, N Hou, M Kobayashi, R Torii, M Hosaka, T Kitamura, T Takeuchi, H Gomi The pseudophosphatase phogrin enables glucose-stimulated insulin signaling in pancreatic ?-cells J. Biol. Chem., 2018-02-26;0(0):. 2018-02-26 [PMID: 29483197] (Western Blot, Mouse)
• Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis eLife 10/18/2017 [PMID: 29043977]
• B Hilmarsdot, E Briem, S Halldorsso, J Kricker, S Ingthorsso, S Gustafsdot, GM Mælandsmo, MK Magnusson, T Gudjonsson Inhibition of PTP1B disrupts cell-cell adhesion and induces anoikis in breast epithelial cells Cell Death Dis, 2017-05-11;8(5):e2769. 2017-05-11 [PMID: 28492548] (ICC, Human)
• Kuo CC, Chu CY, Lin JJ, Lo LC Selective activation of SHP2 activity by cisplatin revealed by a novel chemical probe-based assay. Biochem. Biophys. Res. Commun., 2009-11-10;391(1):230-4. 2009-11-10 [PMID: 19909727] (Western Blot, Human)
• Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation. J. Biol. Chem., 2008-10-07;283(50):35265-72. 2008-10-07 [PMID: 18840608] (Immunoprecipitation, Human)
• Lei K, Townsend DM, Tew KD Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response. Oncogene, 2008-05-05;27(36):4877-87. 2008-05-05 [PMID: 18454177] (Immunoprecipitation, Western Blot, Human)
• Cortesio CL, Chan KT, Perrin BJ, Burton NO, Zhang S, Zhang ZY, Huttenlocher A Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion. J. Cell Biol., 2008-03-10;180(5):957-71. 2008-03-10 [PMID: 18332219] (Western Blot, Human)

Review for PTP1B/PTPN1 Antibody (AF1366) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22282656
• Species: Human

Bioinformatics

• Gene Symbol: PTPN1