PTP1B/PTPN1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and TF‑1 human erythroleukemic cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified ...read more
PTP1B was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) at 15 µg/mL ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 57 kDa (as indicated) using 10 µg/mL of Rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

PTP1B/PTPN1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PTP1B
Specificity
Detects endogenous human PTP1B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF1366 in the following applications:

Publications
Read Publications using
AF1366 in the following applications:

  • ICC
    1 publication
  • IP
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP1B/PTPN1 Antibody [Unconjugated]

  • EC 3.1.3.48
  • protein tyrosine phosphatase 1B
  • protein tyrosine phosphatase, non-receptor type 1
  • Protein-tyrosine phosphatase 1B
  • PTP1B
  • PTP-1B
  • PTP1Bprotein tyrosine phosphatase, placental
  • PTPN1
  • tyrosine-protein phosphatase non-receptor type 1

Background

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTP1B/PTPN1 Antibody (AF1366)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IP, WB.


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ICC
(1)
IP
(2)
WB
(2)
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Human
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Review for PTP1B/PTPN1 Antibody (AF1366) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1366:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22282656
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PTP1B/PTPN1 Antibody (AF1366) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for PTP1B/PTPN1 Antibody (AF1366)

Discover related pathways, diseases and genes to PTP1B/PTPN1 Antibody (AF1366). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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Anonymous
01/05/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol PTPN1
Entrez