PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

Western Blot: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis using PTPN1 mouse mAb against Jurkat (1), MCF-7 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis of Hela cells using PTPN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis of paraffin-embedded bladder cancer tissues using PTPN1 mouse mAb with DAB staining.

Flow Cytometry: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis of Jurkat cells using PTPN1 mouse mAb (green) and negative control (red).

Western Blot: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis using PTPN1 mAb against HEK293 (1) and PTPN1 (AA: 40-246)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis using PTPN1 mAb against human PTPN1 (AA: 40-246) recombinant protein. (Expected MW is 50 kDa)

ELISA: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 4F8F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PTP1B/PTPN1 Antibody (4F8F11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PTP1B/PTPN1 (AA: 40-246) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

• EC 3.1.3.48
• protein tyrosine phosphatase 1B
• protein tyrosine phosphatase, non-receptor type 1
• Protein-tyrosine phosphatase 1B
• PTP1B
• PTP-1B
• PTP1Bprotein tyrosine phosphatase, placental
• PTPN1
• tyrosine-protein phosphatase non-receptor type 1

Background

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN1