PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

Immunoprecipitation: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Characterization of purified monoclonal antibodies targeting RPTP sigma.COS7 cells were transfected with either human RPTP sigma (hRPTP sigma) or mouse RPTP sigma (HA-mRPTPs) and 24 hrs post-transfection, immunoprecipitation (B) analysis was performed. Antibody 1H6 reacted with both forms was used as positive control. Actin was the loading controls. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0178489), licensed under a CC-BY license.

Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - PTPRS monoclonal antibody (M01), clone 1H6 Western Blot analysis of PTPRS expression in HeLa ( Cat # L013V1 ).

Sandwich ELISA: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Detection limit for recombinant GST tagged PTPRS is 0.03 ng/ml as a capture antibody.

Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Characterization of purified monoclonal antibodies targeting RPTP sigma .COS7 cells were transfected with either human RPTP sigma (hRPTP sigma ) or mouse RPTP sigma (HA-mRPTPs) & 24 hrs post-transfection, western blot (A) & immunoprecipitation (B) analysis was performed using the indicated purified monoclonal antibodies. Antibody 1H6 reacted with both forms was used as positive control. Actin was the loading controls. C: Differentiated SH-SY5Y cells were immunostained using the purified 1.3H12 or 4.5H5 monoclonal antibodies for RPTP sigma (green), Dapi for the nucleus (blue) & Phalloidin for actin (red). IgG was used as negative control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0178489), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PTPRS (AAH29496, 31 a.a. ~ 128 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EPPRFIKEPKDQIGVSGGVASFVCQATGDPKPRVTWNKKGKKVNSQRFETIEFDESAGAVLRIQPLRTPRDENVYECVAQNSVGEITVHAKLTVLRGP
• Localization: Cell Membrane; Single-pass type I membrane protein.
• Specificity: PTPRS - protein tyrosine phosphatase, receptor type, S
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRS
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

• EC 3.1.3.48
• protein tyrosine phosphatase PTPsigma
• protein tyrosine phosphatase, receptor type, S
• protein tyrosine phosphatase, receptor type, sigma
• PTP sigma
• PTPR sigma
• PTPRS
• PTPSIGMA
• receptor-type tyrosine-protein phosphatase S
• Receptor-type tyrosine-protein phosphatase sigma
• R-PTP-S
• R-PTP-sigma

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP sigma/PTPRS Antibody (H00005802-M01)(1)

Publication using H00005802-M01

• Wu CL, Hardy S, Aubry I et al. Identification of function-regulating antibodies targeting the receptor protein tyrosine phosphatase sigma ectodomain. PLoS One 2017-05-30 [PMID: 28558026]

Bioinformatics

• Gene Symbol: PTPRS
• Entrez:
  • 5802(Human)
• OMIM:
  • 601576(Human)
• Uniprot:
  • AAH29496(Human)