PTP mu/PTPRM Antibody

Immunohistochemistry-Paraffin: PTP mu/PTPRM Antibody [NB100-79867] - (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PTP mu/PTPRM Antibody [NB100-79867] - (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PTP mu/PTPRM Antibody Summary

• Description: Novus Biologicals Goat PTP mu/PTPRM Antibody (NB100-79867) is a polyclonal antibody validated for use in IHC and ELISA. Anti-PTP mu/PTPRM Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KLIRQVDKWQEEYN corresponding to internal region according to NP_001098714.1, NP_002836.3.
• Epitope: KLIRQVDKWQEEYN
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001098714.1 and NP_002836.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPRM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave bands at approx. 110 kDa band and 16 kDa band in rat retina lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 165 kDa band according to human NP_001098714.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Reactivity Notes

Expected from sequence similarity: Mouse, Dog

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTP mu/PTPRM Antibody

• EC 3.1.3.48
• hR-PTPu
• MGC166994
• protein tyrosine phosphatase mu
• protein tyrosine phosphatase, receptor type, M
• protein tyrosine phosphatase, receptor type, mu polypeptide
• Protein-tyrosine phosphatase mu
• PTP mu
• PTPRL1
• PTPRL1R-PTP-MU
• PTPRM
• receptor-type tyrosine-protein phosphatase mu
• RPTPM
• R-PTP-mu
• RPTPU

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion. Alternative splicing results in multiple transcripts encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP mu/PTPRM Antibody (NB100-79867)(1)

Publication using NB100-79867

• Aricescu AR, Hon WC, Siebold C et al. Molecular analysis of receptor protein tyrosine phosphatase mu-mediated cell adhesion. EMBO J 2006-02-22 [PMID: 16456543]

Bioinformatics

• Gene Symbol: PTPRM
• Entrez:
  • 5797(Human)
  • 19274(Mouse)
• Uniprot:
  • NP_001098714.1(Human)