RGS5 Antibody (OTI1C1)

Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin and eosin and regulator of G-protein signaling (RGS) 5 in squamous cell carcinoma of the tongue in noninvasive and invasive portions. (a), (b), show H&E and RGS5 in noninvasive portions at 10x magnification. (f), (g), show H&E and RGS5 in noninvasive portions at 40x magnification. (k), (l) show H&E and RGS5 in invasive portions at 10x magnification. (p), (q), show H&E and RGS5 in invasive portions at 40x magnification. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1002/cre2.166) licensed under a CC-BY license.

Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.

Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min)

Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RGS5 antibody and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RGS5 Antibody (OTI1C1) Summary

• Immunogen: Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RGS5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RGS5 Antibody (OTI1C1)

• MST092
• MST106
• MST129
• MSTP032
• MSTP092
• MSTP106
• MSTP129
• regulator of G-protein signaling 5
• regulator of G-protein signalling 5

Background

This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGS5 Antibody (NBP2-00880)(4)

Publications using NBP2-00880

• Kondo R, Akiba J, Ogasawara S et al. Programmed death-ligand 1 expression is an unfavorable prognostic factor of hepatocellular carcinoma after archiving sustained virologic response for hepatitis C virus infection Oncol Lett 2019-08-01 [PMID: 31423211] (IF/IHC, Human)
• Li X, Guo W, Zha K et al. Enrichment of CD146+ Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration Theranostics 2019-07-09 [PMID: 31410204] (WB, Human)
• Abe Y, Ogasawara S, Akiba J, Naito Y et al. Expression and role of regulator of G-protein signaling 5 in squamous cell carcinoma of the tongue Clin Exp Dent Res 2019-04-01 [PMID: 31049219] (IHC-P, Human)
• Umeno Y, Ogasawara S, Akiba J, Hattori S. Regulator of G-protein signaling 5 enhances portal vein invasion in hepatocellular carcinoma. Oncol Lett. 2018-02-01 [PMID: 29434872] (IHC-P, Human)

FAQs for RGS5 Antibody (NBP2-00880). (Showing 1 - 1 of 1 FAQs).

1. Could you tell us any information of epitope?
   • About NBP2-00880 (RGS5 Antibody/1C1), it is a monoclonal mouse anti-human RGS5 antibody that was raised again the full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell. Therefore the narrow epitope could be anywhere in the full length protein. Unfortunately, our labs do not map the epitopes for any of our antibodies, including the monoclonal ones.

Bioinformatics

• Gene Symbol: RGS5
• Entrez:
  • 8490(Human)