TUSC3 Antibody

Western Blot: TUSC3 Antibody [NBP1-00248] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TUSC3 Antibody [NBP1-00248] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.

Immunohistochemistry-Paraffin: TUSC3 Antibody [NBP1-00248] - Staining of paraffin embedded Human Prostate at dilution of 8ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TUSC3 Antibody Summary

• Immunogen: Peptide with sequence PQRQCSVCRQAN corresponding to internal region according to NP_839952.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_006756.2; NP_839952.1).
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TUSC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 - 8 ug/mL
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 37 kDa band observed in human liver lysates (calculated MW of 39.7 kDa band according to NP_006756.2 and of 39.6 kDa band according to NP_839952.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  TUSC3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TUSC3 Antibody

• D8S1992
• M33
• N33MRT7MGC13453
• oligosaccharyltransferase 3 homolog A
• OST3A
• Protein N33
• Putative prostate cancer tumor suppressor
• tumor suppressor candidate 3

Background

This gene is a candidate tumor suppressor gene. It is located within a homozygously deleted region of a metastatic prostate cancer. The gene is expressed in most nonlymphoid human tissues including prostate, lung, liver, and colon. Expression was also detected in many epithelial tumor cell lines. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TUSC3 Antibody (NBP1-00248)(1)

Publication using NBP1-00248

• Pils D, Horak P, Gleiss A et al. Five genes from chromosomal band 8p22 are significantly down-regulated in ovarian carcinoma: N33 and EFA6R have a potential impact on overall survival. Cancer 2005-12-01 [PMID: 16270321]

Bioinformatics

• Gene Symbol: TUSC3
• Entrez:
  • 7991(Human)
  • 80286(Mouse)
  • 290783(Rat)
• Uniprot:
  • NP_006756.2(Human)