LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EDG2.

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EDG2 antibody, and then analyzed by flow cytometry.

Analysis of overexpressedlysates (25ug per lane) from HEK293T cellstransfected with empty plasmid, lane1) , human LPAR1 plasmid ,lane 2),mouse LPAR1 plasmid ([MR205601], lane 3), ratLPAR1 plasmid, lane 4) using antiLPAR1 antibody (1:500). Figure B,Western blot analysis of the same samples asfigure A with anti-DDK antibody,1:1000)

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.8 mg/ml

LPAR1/LPA1/EDG-2 Antibody (OTI1G6) Summary

• Description: Novus Biologicals Mouse LPAR1/LPA1/EDG-2 Antibody (OTI1G6) (NBP2-01354) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. Anti-LPAR1/LPA1/EDG-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human LPAR1(NP_001392) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LPAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 40.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.8 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

• EDG2
• edg-2
• EDG2Lysophosphatidic acid receptor Edg-2
• endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,
• GPCR26
• GPR26
• LPA receptor 1
• LPA1
• LPA-1
• LPA1vzg-1
• LPAR1
• lysophosphatidic acid receptor 1
• Mrec1.3
• rec.1.3
• ventricular zone gene 1
• VZG1
• vzg-1

Background

The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LPAR1/LPA1/EDG-2 Antibody (NBP2-01354)(1)

Publication using NBP2-01354

• Pan H L, Liu B L et al Modulation of Nav1.8 by Lysophosphatidic Acid in the Induction of Bone Cancer Pain Neurosci Bull 2016-09-16 [PMID: 27631681] (WB, IF/IHC, Rat)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Bioinformatics

• Gene Symbol: LPAR1
• Entrez:
  • 1902(Human)