PTP-MEG2/PTPN9 Antibody (291835) [Unconjugated]

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, TS1 mouse helper T cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PTP-MEG2 Monoclonal Antibody (Catalog # MAB2668) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTP-MEG2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 291835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PTP-MEG2/PTPN9 Antibody (291835) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PTP-MEG2
  Glu2-Gln593
  Accession # P43378
• Specificity: Detects human, mouse, and rat PTP-MEG2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP-MEG2/PTPN9 Antibody (291835) [Unconjugated]

• EC 3.1.3.48
• MEG2
• protein tyrosine phosphatase, non-receptor type 9
• Protein-tyrosine phosphatase MEG2
• PTPase MEG2
• PTPase-MEG2
• PTPMEG2
• PTP-MEG2
• PTPN9
• tyrosine-protein phosphatase non-receptor type 9

Background

Megakaryocyte protein tyrosine phosphatase 2 (PTP-MEG2), also known as PTPN9, contains an N-terminal domain homologous to the yeast retinaldehyde binding domain Sec14p and a C-terminal phosphatase domain. The Sec14p domain targets PTP-MEG2 to the membrane of secretory vesicles, where it promotes vesicle fusion. Elevated levels of membrane-bound PTP-MEG2 activity in erythroid progenitor cells are observed in polycythemia vera and may affect responsiveness to colony stimulating factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP-MEG2/PTPN9 Antibody (MAB2668)(6)

Publications using MAB2668

• X Chen, W Liu, X Guo, S Huang, X Song Ropivacaine inhibits cervical cancer cell growth via suppression of the miR?96/MEG2/pSTAT3 axis Oncol. Rep., 2020-02-26;43(5):1659-1668. 2020-02-26 [PMID: 32323811] (Western Blot, Human)
• Z Liu, F Sun, Y Hong, Y Liu, M Fen, K Yin, X Ge, F Wang, X Chen, W Guan MEG2 is regulated by miR-181a-5p and functions as a tumour suppressor gene to suppress the proliferation and migration of gastric cancer cells Mol. Cancer, 2017-07-26;16(1):133. 2017-07-26 [PMID: 28747184] (Western Blot, Human)
• miR-96 promotes cell proliferation, migration and invasion by targeting PTPN9 in breast cancer Sci Rep, 2016-11-18;6(0):37421. 2016-11-18 [PMID: 27857177] (Western Blot, Human)
• Guangpu Li The Protein Tyrosine Phosphatase MEG2 Regulates the Transport and Signal Transduction of Tropomyosin Receptor Kinase A J. Biol. Chem., 2016-09-21;291(46):23895-23905. 2016-09-21 [PMID: 27655914] (ICC, Western Blot, Mouse, Rat)
• Huang X, Gschweng E, Van Handel B Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells. Blood, 2010-12-16;117(7):2157-65. 2010-12-16 [PMID: 21163928] (Western Blot, Human)
• Hao Q, Wang L, Zhao ZJ, Tang H Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis. J. Biol. Chem., 2008-11-09;284(2):799-806. 2008-11-09 [PMID: 19001381] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PTPN9
• Uniprot:
  • P43378(Mouse)