PTP epsilon Antibody (OTI5F2) - Azide and BSA Free

Western Blot: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTP epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTP epsilo

Immunocytochemistry/Immunofluorescence: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP epsilon.

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded liver tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded Kidney tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded prostate tissue using anti-PTP epsilon mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI5F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PTP epsilon Antibody (OTI5F2) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 80.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PTP epsilon Antibody (OTI5F2) - Azide and BSA Free

• DKFZp313F1310
• EC 3.1.3.48
• FLJ57799
• FLJ58245
• HPTPE
• protein tyrosine phosphatase epsilon
• protein tyrosine phosphatase, receptor type, E
• protein tyrosine phosphatase, receptor type, epsilon polypeptide
• Protein-tyrosine phosphatase epsilon
• PTPE
• receptor-type tyrosine-protein phosphatase epsilon
• R-PTP-EPSILON

Background

PTP epsilon, an R4 receptor protein tyrosine phosphatase, is composed of a short extracellular and two cyplasmic protein phosphatase domains and has been shown to affect neuronal differentiation, endothelial cell growth, vascular development, and possibly mammary tumor development. It has been shown that the cytosolic form of PTP epsilon can be induced by IL-1 and TNF alpha in humans and is a negative regulator of IL-6- and LIF-induced Jak-STAT kinase signaling in rats. Four alternatively spliced protein isoforms have been documented for PTP epsilon: a membrane form, a cytosolic form, the p65 form, which results from translation using an internal initiation codon, and the p67 form, which results from a specific proteolytic cleavage of wild-type PTP epsilon. PTP epsilon expression has been documented in human vascular endothelial cells, astrocytoma cells, and in IW32 erythroleukemia overexpressing p53. PTP epsilon expression has been documented in animal brain, breast, ganglion, heart, kidney, liver, lung, nerve, spinal cord, spleen, testis, and vessel. The membrane and cytosolic forms of PTP epsilon show different tissue expression patterns. ESTs have been isolated from numerous human tissue libraries, including normal human blood, brain, testis, and thyroid, and cancerous human blood, brain, breast, colon, embryo, head/neck, pancreas, and skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRE