PTP epsilon Antibody (OTI5F2)

Western Blot: PTP epsilon Antibody (5F2) [NBP2-02374] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTP epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTP epsilon.

Immunocytochemistry/Immunofluorescence: PTP epsilon Antibody (5F2) [NBP2-02374] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP epsilon.

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded prostate tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded Kidney tissue using anti-PTP epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded liver tissue using anti-PTP epsilon mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI5F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.58 mg/ml

PTP epsilon Antibody (OTI5F2) Summary

• Description: Novus Biologicals Mouse PTP epsilon Antibody (OTI5F2) (NBP2-02374) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 80.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.58 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTP epsilon Antibody (OTI5F2)

• DKFZp313F1310
• EC 3.1.3.48
• FLJ57799
• FLJ58245
• HPTPE
• protein tyrosine phosphatase epsilon
• protein tyrosine phosphatase, receptor type, E
• protein tyrosine phosphatase, receptor type, epsilon polypeptide
• Protein-tyrosine phosphatase epsilon
• PTPE
• receptor-type tyrosine-protein phosphatase epsilon
• R-PTP-EPSILON

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported,one of which encodes a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region,and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokines induced SATA signaling, as well as the activation of voltage-gated K+ channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRE
• Entrez:
  • 5791(Human)