PTEN Antibody

Western Blot: PTEN Antibody [NBP1-51919] - staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

PTEN Antibody Summary

• Immunogen: internal region (near the C Terminus)
• Epitope: CNEPFDEDQHTQIT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTEN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:32000
  • Western Blot 1-3 ug/ml
• Application Notes: Approx 55kDa band observed in lysates of cell line A431 and 50kDa in lysates of cell line NIH3T3 (calculated MW of 47.1kDa according to Human NP_000305 and Mouse NP_032986.1). Recommended concentration: 1-3ug/ml.

Reactivity Notes

Expected from sequence similarity: Rat, Dog, Pig and Cow.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

On restricted sale due to demands of patent holder. Not for sale in USA, if you are outside the USA then please contact Novus to see if we can sell into your territory.

Alternate Names for PTEN Antibody

• BZS
• EC 3.1.3.16,10q23del
• EC 3.1.3.48
• EC 3.1.3.67
• GLM2
• MMAC1 phosphatase and tensin homolog deleted on chromosome 10
• MMAC1
• MMAC1MGC11227
• Mutated in multiple advanced cancers 1
• phosphatase and tensin homologDEC
• phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN
• PTEN
• PTEN1
• TEP1MHAM

Background

PTEN functions primarily as a lipid phosphatase to regulate crucial signal transduction pathways; a key target is phosphatidylinositol 3,4,5 trisphosphate. In addition, it displays weak tyrosine phosphatase activity, which may down modulate signaling pathways that involve focal adhesion kinase or Shc. PTEN negatively regulates Akt activation by preventing its phosphorylation. PTEN therefore inhibits the PI 3 kinase/Akt signaling pathway, which is important for cell growth and survival. Mutations of PTEN occur in 60 to 80% of prostate cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTEN Antibody (NBP1-51919)(1)

Publication using NBP1-51919

• Drinjakovic J, Jung H, Campbell DS et al. E3 ligase Nedd4 promotes axon branching by downregulating PTEN. Neuron. 2010-02-11 [PMID: 20159448] (WB)
  
  Details:
  WB (Xenopus embryo retinas injected with PTEN or control antisense morpholinos), Fig 7A. PTEN was detected at ~ 52 kDa. An additional, uncharacterized band was also detected slightly above the 38 kDa marker. Note: The specificity of the PTEN (IMG-3386) pA

Bioinformatics

• Gene Symbol: PTEN
• Entrez:
  • 5728(Human)
• Uniprot:
  • NP_000305.3(Human)