PSTPIP1 Antibody (1D5) - Azide and BSA Free

Western Blot: PSTPIP1 Antibody (1D5) [H00009051-M02] - PSTPIP1 monoclonal antibody (M02), clone 1D5. Analysis of PSTPIP1 expression in Raw 264.7.

ELISA: PSTPIP1 Antibody (1D5) [H00009051-M02] - Detection limit for recombinant GST tagged PSTPIP1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 1D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PSTPIP1 Antibody (1D5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSTPIP1 (AAH08602, 1 a.a. ~ 416 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASFDSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIAVQEIQGNPASPAQEYRALYDYTAQNPDELDLSAGDILEVILEGEDGWWTVERNGQRGFVPGSYLEKL
• Specificity: Reacts with proline-serine-threonine phosphatase interacting protein 1.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSTPIP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PSTPIP1 Antibody (1D5) - Azide and BSA Free

• CD2BP1CD2-binding protein 1
• CD2BP1L
• CD2BP1S
• H-PIPPEST phosphatase-interacting protein 1
• PAPASCD2 antigen-binding protein 1
• proline-serine-threonine phosphatase interacting protein 1
• proline-serine-threonine phosphatase-interacting protein 1
• PSTPIPCD2 cytoplasmic tail-binding protein

Background

The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSTPIP1 Antibody (H00009051-M02)(2)

Publications using H00009051-M02

• Moghaddas F, Llamas R, De Nardo D et al. A novel Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis mutation further defines 14-3-3 binding of pyrin and distinction to Familial Mediterranean Fever. Ann Rheum Dis 2017-08-23 [PMID: 28835462]
• Sztacho M, Segeletz S, Sanchez-Fernandez MA et al. BAR Proteins PSTPIP1/2 Regulate Podosome Dynamics and the Resorption Activity of Osteoclasts. PLoS One 2016-10-19 [PMID: 27760174]

Bioinformatics

• Gene Symbol: PSTPIP1
• Entrez:
  • 9051(Human)
• Uniprot:
  • AAH08602(Human)