PSMB10/MECL1 Antibody (S06-6C5)

Western Blot: PSMB10/MECL1 Antibody (S06-6C5) [NBP3-19751] - Western blot detection of PSMB10/MECL1 in C6 cell lysates using NBP3-19751 (1:500 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: S06-6C5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PSMB10/MECL1 Antibody (S06-6C5) Summary

• Description: Novus Biologicals Rabbit PSMB10/MECL1 Antibody (S06-6C5) (NBP3-19751) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PSMB10/MECL1 (Uniprot # P40306)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PSMB10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PSMB10/MECL1 Antibody (S06-6C5)

• beta2i
• FLJ00366
• LMP10
• LMP10Proteasome subunit beta-2i
• Low molecular mass protein 10
• Macropain subunit MECl-1
• MECL1
• MECl-1
• MECL1EC 3.4.25.1
• MGC1665
• Multicatalytic endopeptidase complex subunit MECl-1
• proteasome (prosome, macropain) subunit, beta type, 10
• proteasome catalytic subunit 2i
• Proteasome MECl-1
• proteasome subunit beta 7i
• proteasome subunit beta type-10
• Proteasome subunit beta-2i
• proteasome subunit MECL1
• PSMB10

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMB10