Western Blot: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of PSMB8 expression in transfected 293T cell line by PSMB8 monoclonal antibody (M01), clone 1B3.Lane 1: PSMB8 transfected lysate(29.8 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of monoclonal antibody to PSMB8 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Immunoprecipitation: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of PSMB8 transfected lysate using anti-PSMB8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMB8 MaxPab rabbit ...read more
ELISA: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Detection limit for recombinant GST tagged PSMB8 is approximately 1ng/ml as a capture antibody.
PSMB8 (AAH01114, 173 a.a. ~ 272 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our LMP7/PSMB8 Antibody (1B3) and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.