PSGL-1/CD162 Antibody (688101) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
688101
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

PSGL-1/CD162 Antibody (688101) [Alexa Fluor® 750] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PSGL-1/CD162
Gln42-Gly295
Accession # NP_002997
Specificity
Detects human PSGL-1/CD162 in direct ELISAs.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SELPLG
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PSGL-1/CD162 Antibody (688101) [Alexa Fluor® 750]

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

Human PSGL-1 (P-Selectin Glycoprotein Ligand-1; also CD162), is a 120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). It is synthesized as a 412 amino acid (aa) preproprecursor that contains a 17 aa signal sequence, a 24 aa propeptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (4, 5). Following cleavage of the pre- and prosegments, it is expressed as a 240 kDa disulfide-linked homodimer. The extreme N-terminus (aa 1-16 of the mature molecule) contains one threonine (aa 16) and three tyrosines (aa 5, 7, and 10) that are involved in ligand binding. The Thr residue allows for O-linked glycosylation in the form of a core-2 structure (GalNAc-Gal) linked in a beta 1,6 bond to a sialylated Lewis X motif (GlcNAc linked to both Fuc and Gal with a terminal sialic acid residue) (1, 2, 5, 6, 7). The three tyrosine residues allow for sulfation (8, 9). When binding to P-selectin, Tyr sulfation and glycosylation are essential. Tyr7 provides the most efficient sulfate moiety, while Fuc and sialic acid are essentially mandatory (7). When binding to E-selectin, only carbohydrate is needed, while both carbohydrate and Tyr10 are used for L-selectin binding (6, 8). There are 16 decameric aa repeats in the ECD of the longform of PSGL-1. This form is referred to as the A allele, and represents 65 - 80% of the population. Alleles B and C show deletions of decameric repeats #2 (aa 132-141) plus #9 and 10 (aa 222-241), respectively. Shorter forms may show weaker binding to P-selectin (9, 10). Soluble forms of PSGL-1 are also known. Neutrophil elastase will cleave somewhere within repeats #5-9, while cathepsin G cleaves after Tyr7 (11). The loss of Tyr5 and 7 should impact binding affinity. PSGL-1 is found on virtually all leukocytes and macrophages/DC’s (1). Although there is similarity in the organization of the ECD between species, there is little aa identity. Human PSGL-1 ECD shares 51%, 52% and 43% aa sequence identity with equine, canine and mouse ECD, respectively.

  1. Yang, J. et al. (1999) Thromb. Haemost. 81:1.
  2. Cummings, R.D. (1999) Braz. J. Med. Biol. Res. 32:519.
  3. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
  4. Sako, D. et al. (1993) Cell 75:1179.
  5. Veldman, G.M. et al. (1995) J. Biol. Chem. 270:16470.
  6. Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.
  7. Leppanen, A. et al. (2000) J. Biol. Chem. 275:39569.
  8. Sako, D. et al. (1995) Cell 83:323.
  9. Afshar-Kharghan, V. et al. (2001) Blood 97:3306.
  10. Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.
  11. Gardiner, E.E. et al. (2001) Blood 98:1440.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SELPLG
Uniprot