Prox1 Antibody (CL13472) - Azide and BSA Free

Staining of human duodenum shows moderate nuclear positivity in a subset of glandular cells.

Staining of human pancreas shows weak to moderate nuclear positivity in exocrine glandular cells.

Staining of human skin shows no positivity in squamous epithelial cells as expected.

Staining of human prostate shows no positivity in glandular cells as expected.

Staining of mouse hippocampus shows strong nuclear positivity in the granular cell layer of the dentate gyrus.

Analysis in human cell line SH-SY5Y.

Staining of rat hippocampus shows strong nuclear positivity in the granular cell layer of the dentate gyrus.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL13472
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Prox1 Antibody (CL13472) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Prox1 Antibody (CL13472) - Azide and BSA Free (NBP3-24560) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q92786, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PROX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Prox1 Antibody (CL13472) - Azide and BSA Free

• Homeobox prospero-like protein PROX1
• prospero homeobox 1
• prospero homeobox protein 1
• prospero-related homeobox 1
• Prox1
• PROX-1

Background

PROX1 may play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons. PROX1 antibodies are also used as a highly specific marker of lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PROX1