Protein S/PROS1 Antibody (818002)

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4976) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein S/PROS1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 818002
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Protein S/PROS1 Antibody (818002) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Protein S/PROS1
  Met1-Arg669
  Accession # Q08761
• Specificity: Detects mouse Protein S/PROS1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protein S is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PROS1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein S/PROS1 Antibody (818002)

• EC 3.4.21
• PROS
• PROS1
• protein S (alpha)
• Protein S
• protein Sa
• PS21
• PS22
• PS23
• PS24
• PS25
• PSA
• vitamin K-dependent plasma protein S
• vitamin K-dependent protein S

Background

Protein S (PROS1) is a 70-75 kDa plasma glycoprotein member of the vitamin K dependent family of proteins.  It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium.  Protein S is a non-enzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa.  It also binds to Axl on smooth muscle, likely initiating mitosis.  Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP.  Mouse Protein S precursor is 675 amino acids (aa) in length.  It contains a signal sequence (aa 1-24), a cleavable prosegment (aa 25-41) and a 634 aa mature region.  The mature molecule contains one Gla region (aa 42-87), four EGF-like domains (aa 117-283) and two laminin G-like regions (aa 299-665).  Over aa 1-169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protein S/PROS1 Antibody (MAB4976)(2)

Publications using MAB4976

• ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature, 2022-06-01;606(7913):396-405. 2022-06-01 [PMID: 35650435] (Western Blot, Mouse)
• WE Plautz, VS Sekhar Pil, BC Cooley, R Chattopadh, PR Westmark, T Getz, D Paul, W Bergmeier, JP Sheehan, R Majumder Anticoagulant Protein S Targets the Factor IXa Heparin-Binding Exosite to Prevent Thrombosis Arterioscler. Thromb. Vasc. Biol., 2018-02-01;0(0):. 2018-02-01 [PMID: 29419409] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PROS1
• Uniprot:
  • Q08761()