Protein Phosphatase 1 beta Antibody - BSA Free

Western Blot: Protein Phosphatase 1 beta Antibody [NBP2-49446] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue

Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP2-49446] - Staining of human cell line RH-30 shows localization to nucleus & plasma membrane.

Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP2-49446] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Protein Phosphatase 1 beta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Protein Phosphatase 1 beta Antibody - BSA Free (NBP2-49446) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: SEKKAKYQYGGLNSGRPVTPPRTANPP
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1CB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Protein Phosphatase 1 beta Recombinant Protein Antigen (NBP2-49446PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Protein Phosphatase 1 beta Antibody - BSA Free

• EC 3.1.3.16
• EC 3.1.3.53
• MGC3672
• PP1beta
• PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform
• PPP1CD
• protein phosphatase 1, catalytic subunit, beta isoform
• protein phosphatase 1, catalytic subunit, beta isozyme
• protein phosphatase 1-beta
• protein phosphatase 1-delta
• serine/threonine protein phosphatase PP1-beta catalytic subunit
• serine/threonine-protein phosphatase PP1-beta catalytic subunit

Background

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1CB