PPP1R9B/Spinophilin Antibody

Images

 
Western blot shows lysates of human brain tissue and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Rat PPP1R9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6465) followed by ...read more
PPP1R9B was detected in immersion fixed rat hippocampal neurons (E18) using Sheep Anti-Human/Rat PPP1R9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6465) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

PPP1R9B/Spinophilin Antibody Summary

Immunogen
E. coli-derived recombinant human PPP1R9B
Lys289-Asp412
Accession # Q96SB3
Specificity
Detects human and rat PPP1R9B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PPP1R9B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PPP1R9B/Spinophilin Antibody

  • FLJ30345
  • neurabin II
  • Neurabin-2
  • neurabin-II
  • PPP1R6
  • PPP1R9
  • PPP1R9B
  • Protein phosphatase 1 regulatory subunit 9B
  • protein phosphatase 1, regulatory (inhibitor) subunit 9B
  • protein phosphatase 1, regulatory subunit 9B, spinophilin
  • SPINO
  • Spinophilin
  • Spn

Background

PPP1R9B (Protein phosphatase 1 regulatory subunit 9B; also spinophilin and neurabin-II) is a widely expressed intracellular protein that is found in both cytoplasm and nucleus. Although its predicted MW is 89 kDa, it runs anolamously at 120-140 kDa in SDS-Page. PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells. In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules. Human PPP1R9B is 815 amino acids (aa) in length. It contains one actin-binding region (aa 1-154), a PP1 binding domain (aa 415-492), a PZD domain (aa 493-583) and a coiled-coil region (aa 647-815). PPP1R9B is reported to form homotrimers and homotetramers. There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576. Over aa 291-414, human PPP1R9B shares 91% aa identity with mouse PPP1R9B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-77417
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
NBP1-31348
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NLS1071
Species: Hu
Applications: IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-45897
Species: Hu
Applications: WB, IHC
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NB300-653
Species: Hu, Mu, Rt, Ch, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
MAB4077
Species: Hu
Applications: WB, IHC
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow

Publications for PPP1R9B/Spinophilin Antibody (AF6465) (0)

There are no publications for PPP1R9B/Spinophilin Antibody (AF6465).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PPP1R9B/Spinophilin Antibody (AF6465) (0)

There are no reviews for PPP1R9B/Spinophilin Antibody (AF6465). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PPP1R9B/Spinophilin Antibody (AF6465) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PPP1R9B/Spinophilin Products

Bioinformatics Tool for PPP1R9B/Spinophilin Antibody (AF6465)

Discover related pathways, diseases and genes to PPP1R9B/Spinophilin Antibody (AF6465). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PPP1R9B/Spinophilin Antibody (AF6465)

Discover more about diseases related to PPP1R9B/Spinophilin Antibody (AF6465).
 

Pathways for PPP1R9B/Spinophilin Antibody (AF6465)

View related products by pathway.

PTMs for PPP1R9B/Spinophilin Antibody (AF6465)

Learn more about PTMs related to PPP1R9B/Spinophilin Antibody (AF6465).

Blogs on PPP1R9B/Spinophilin

There are no specific blogs for PPP1R9B/Spinophilin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PPP1R9B/Spinophilin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PPP1R9B
Entrez
Uniprot