Protein Disulfide Isomerase/P4HB Overexpression Lysate (Native)

Images

 
Western Blot: P4HB Overexpression Lysate (Adult Normal) [NBP2-04183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P4HB.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Protein Disulfide Isomerase/P4HB Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000909. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens P4HB, mRNA.
Gene
P4HB

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Protein Disulfide Isomerase/P4HB

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Protein Disulfide Isomerase/P4HB Overexpression Lysate (Native)

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89394
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-13883
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MAB224
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
NB110-40561
Species: Hu
Applications: WB, IP
AF5789
Species: Hu, Mu, Rt
Applications: WB
NBP1-18910
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1138
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-49833
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183) (0)

There are no publications for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183) (0)

There are no reviews for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Protein Disulfide Isomerase/P4HB Products

Bioinformatics Tool for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183)

Discover related pathways, diseases and genes to Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183)

Discover more about diseases related to Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183).
 

Pathways for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183)

View related products by pathway.

PTMs for Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183)

Learn more about PTMs related to Protein Disulfide Isomerase/P4HB Lysate (NBP2-04183).

Blogs on Protein Disulfide Isomerase/P4HB

There are no specific blogs for Protein Disulfide Isomerase/P4HB, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Protein Disulfide Isomerase/P4HB Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol P4HB
Entrez