Protein Disulfide Isomerase/P4HB Antibody (RL77)

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Protein Disulfide Isomerase/P4HB using monoclonal antibody shows staining in A549 Cells.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.

Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 3T3 cells compared to an isotype control (blue).

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Staining of rat epithelial cells.

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of NS-1 Cells.

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis shows staining in HMVEC Cells.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Normal deparaffinized Human tonsil tissue tissues.

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human liver tissues.

Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of K562 cells compared to an isotype control (blue).

Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Hela cells compared to an isotype control (blue).

• Reactivity: Hu, Mu, Rt, Po, Ha, Xp
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: RL77
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Protein Disulfide Isomerase/P4HB Antibody (RL77) Summary

• Description: Novus Biologicals Mouse Protein Disulfide Isomerase/P4HB Antibody (RL77) (NB600-1164) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-Protein Disulfide Isomerase/P4HB Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified rat Protein Disulfide Isomerase/P4HB protein.
• Localization: Endoplasmic reticulum
• Marker: Endoplasmic Reticulum Marker
• Specificity: NB600-1164 detects Protein Disulfide Isomerase/P4HB from human, mouse, rat, porcine, xenopus liver tissues as well as hamster (CHO) cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: P4HB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:200 - 1:2000
• Application Notes: WB usage was reported in scientific literature (PMID: 21292774). WB: Detects an approx. 59 kDa representing Protein Disulfide Isomerase/P4HB from rat liver extract or a slightly higher protein at 61 kDa representing Protein Disulfide Isomerase/P4HB from human liver extract. IHC: Staining of Protein Disulfide Isomerase/P4HB in rat intestine with ab5484 yields a pattern consistent with cytoplasmic staining. IP: ab5484 has been shown to inhibit the activity of Protein Disulfide Isomerase/P4HB in vitro.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33806982).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody (RL77)

• Cellular thyroid hormone-binding protein
• collagen prolyl 4-hydroxylase beta
• DSI
• ERBA2L
• GIT
• glutathione-insulin transhydrogenase
• P4HB
• P4Hbeta
• p55
• PDI
• PDIA1
• PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
• PDIEC 5.3.4.1
• PHDB
• PO4DB
• PO4HB
• procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
• PROHB
• PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
• Prolyl 4-hydroxylase subunit beta
• prolyl 4-hydroxylase, beta polypeptide
• protein disulfide isomerase family A, member 1
• protein disulfide isomerase/oxidoreductase
• protein disulfide isomerase-associated 1
• protein disulfide-isomerase
• protocollagen hydroxylase
• Thbp
• thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protein Disulfide Isomerase/P4HB Antibody (NB600-1164)(3)

Publications using NB600-1164

• Gaspar R, Sage T, Little G et al. Protein Disulphide Isomerase and NADPH Oxidase 1 Cooperate to Control Platelet Function and Are Associated with Cardiometabolic Disease Risk Factors Antioxidants 2021-03-23 [PMID: 33806982] (ICC/IF, Human)
• Lee S, Min Kim S, Dotimas J et al. Thioredoxin-interacting protein regulates protein disulfide isomerases and endoplasmic reticulum stress. EMBO Mol Med. 2014-05-19 [PMID: 24843047] (WB, Human)
• Jun HS, Lee YM, Song K et al. G-CSF improves murine G6PC3-deficient neutrophil function by modulating apoptosis and energy homeostasis Blood 2011-04-07 [PMID: 21292774] (WB, Mouse)

FAQs for Protein Disulfide Isomerase/P4HB Antibody (NB600-1164). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of catalog #NB600-1164, lot #MK161542?
   • The concentration would not be relevant for this product as it is not a purified antibody (its an ascites formulation) and our lab informed me that it is "100 ul of ascites fluid (with protein contents 2.3 mg/ml) diluted in PBS with 0.05% sodium azide".

Bioinformatics

• Gene Symbol: P4HB