Proteasome 20S alpha 6 Antibody (S01-4I2)

Western Blot: Proteasome 20S alpha 6 Antibody (S01-4I2) [NBP3-15085] - Western blot detection of Proteasome 20S alpha 6 in K562, C6, 3T3, Hela cell lysates using Proteasome 20S alpha 6 Rabbit mAb (1:1000 diluted). Predicted band size: 27kDa. Observed band size: 27kDa.

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 6 Antibody (S01-4I2) [NBP3-15085] - Immunocytochemistry of Proteasome 20S alpha 6 (green) in Hela using NBP3-15085 at dilution 1/20, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S01-4I2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Proteasome 20S alpha 6 Antibody (S01-4I2) Summary

• Description: Novus Biologicals Rabbit Proteasome 20S alpha 6 Antibody (S01-4I2) (NBP3-15085) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Proteasome 20S alpha 6 (Uniprot#: P60900)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PSMA6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000-1/10000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Proteasome 20S alpha 6 Antibody (S01-4I2)

• EC 3.4.25.1
• IOTA
• Macropain iota chain
• macropain subunit iota
• MGC22756
• MGC2333
• MGC23846
• Multicatalytic endopeptidase complex iota chain
• p27K
• p27KPROS-27
• PROS27
• PROS-27
• PROS2727 kDa prosomal protein
• prosomal P27K protein
• proteasome (prosome, macropain) subunit, alpha type, 6
• Proteasome 20S alpha 6
• Proteasome iota chain
• proteasome subunit alpha type-6
• proteasome subunit iota
• PSMA6

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMA6