Prostatic Acid Phosphatase/ACPP Antibody

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Prostatic Acid Phosphatase/ACPP Antibody Summary

• Immunogen: Peptide with sequence C-YGIHKQKEKSRLQ corresponding to internal region according to NP_001090.2.
• Epitope: C-YGIHKQKEKSRLQ
• Specificity: This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 50 kDa band observed in human prostate lysates is reported in scientific literature (PMID: 3530530). IHC-P: Human prostate shows cytoplasm staining in some secretory cells of the gland.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Prostatic Acid Phosphatase/ACPP Antibody

• acid phosphatase, prostate
• ACP3
• ACP-3
• ACPP
• EC 3.1.3.2
• PAP
• Prostatic Acid Phosphatase
• prostatic acid phosphotase

Background

Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase. This antibody does not inhibit the enzymatic activity of PSAP. It reacts with non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no cross reaction with other phosphatases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prostatic Acid Phosphatase/ACPP Antibody (NBP1-51946)(1)

Publication using NBP1-51946

• Quintero IB, Araujo CL, Pulkka AE et al. Prostatic acid phosphatase is not a prostate specific target. Cancer Res 2007-07-01 [PMID: 17638863]

Bioinformatics

• Gene Symbol: ACP3
• Entrez:
  • 55(Human)
  • 56318(Mouse)
  • 56780(Rat)
• Uniprot:
  • NP_001090.2(Human)