Prostatic Acid Phosphatase/ACPP Antibody

Images

 
Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Prostatic Acid Phosphatase/ACPP Antibody Summary

Immunogen
Peptide with sequence C-YGIHKQKEKSRLQ corresponding to internal region according to NP_001090.2.
Epitope
C-YGIHKQKEKSRLQ
Specificity
This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACPP
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 50 kDa band observed in human prostate lysates is reported in scientific literature (PMID: 3530530). IHC-P: Human prostate shows cytoplasm staining in some secretory cells of the gland.
Publications
Read Publication using NBP1-51946.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Prostatic Acid Phosphatase/ACPP Antibody

  • acid phosphatase, prostate
  • ACP3
  • ACP-3
  • ACPP
  • EC 3.1.3.2
  • PAP
  • Prostatic Acid Phosphatase
  • prostatic acid phosphotase

Background

Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase. This antibody does not inhibit the enzymatic activity of PSAP. It reacts with non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no cross reaction with other phosphatases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prostatic Acid Phosphatase/ACPP Antibody (NBP1-51946)(1)


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Bioinformatics

Gene Symbol ACPP
Entrez
Uniprot