Properdin Antibody (10C5) - BSA Free

Product Discontinued

Properdin Antibody (10C5) - BSA Free Summary

• Immunogen: Properdin isolated from human plasma adsorbed onto aluminum hydroxide gel
• Epitope: Epitope specificity differs from that of Clone 06 but slightly overlap as determined by inhibition ELISA.
• Specificity: Clone 10C5 has specificity for human properdin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 10C5 reacts strongly with properdin isolated from human plasma when tested in sandwich ELISA using Clone 10C5 as capture and biotinylated detection antibody, only very low reaction is seen with plasma from patients deficient in properdin. Clone 10C5 works equally well in ELISA with purified properdin coated directly onto the microtiter well. In Western blotting after SDS-PAGE Clone 10C5 reacts with properdin in both reduced (subunits of 25 kDa and 56 kDa) as well as unreduced forms (220 kDa).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Properdin Antibody (10C5) - BSA Free

• BFD
• CFP
• Complement Factor P
• complement factor properdin
• PFC
• PFCcomplement
• PFD
• Properdin

Background

Properdin in plasma is a mixture of cyclic dimers, trimers and tetramers. The molecular weight of the glycosylated monomer is 53 kDa. Properdin is a regulator protein of the alternative complement pathway. It stabilizes the C3 convertase excerting its effect by binding to C3b in the C3bBb complex and thereby inhibiting cleavage of C3b by factor I and increasing the affinity for factor B. Serum concentration is approximately 25 ug/ml .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFP