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Rat Proinsulin ELISA Kit (Colorimetric)

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Rat Proinsulin ELISA Kit [NBP2-78728] - Standard Curve Reference
Rat Proinsulin ELISA Kit [NBP2-78728] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Rat Proinsulin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat PI in samples. No Significant cross-reactivity or interference between Rat PI and analogues was observed.
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.83%
Intra-Assay
CV% < 6.05%
Spike Recovery
87-107%sampl
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
INS

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Reference Standard & Sample Diluent
  2. Stop Solution
  3. Substrate Reagent
  4. HRP Conjugate Diluent
  5. Micro ELISA Plate(Dismountable)
  6. Product Description
  7. Reference Standard
  8. Plate Sealer
  9. Concentrated HRP Conjugate (100×)
  10. Concentrated Wash Buffer (25×)
  11. Certificate of Analysis
  12. Concentrated Biotinylated Detection Ab (100×)
  13. Biotinylated Detection Ab Diluent

Alternate Names for Rat Proinsulin ELISA Kit (Colorimetric)

  • IDDM2
  • ILPR
  • insulin
  • IRDN
  • MODY10
  • Proinsulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog&amp;nbsp;# MAB10671) coated on a Clear Polystyrene Microplate (Catalog&amp;nbsp;# DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog&amp;nbsp;# DY998) followed by Substrate Solution (Catalog&amp;nbsp;# DY999) and stopping the enzymatic reaction with Stop Solution (Catalog&amp;nbsp;# DY994).
Recombinant Human Growth Hormone (Catalog&amp;nbsp;# 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2&amp;nbsp;ng/mL) is neutralized (green line) by increasing concentrations of Goat&amp;nbsp;Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF1067). The ND50 is typically 1.5-7.5&amp;nbsp;ng/mL.
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Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog&amp;nbsp;# 291-G1) for 15&amp;nbsp;minutes, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
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[Unconjugated]
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(5 Publications)
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Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Gene Symbol INS