Prohibitin Antibody (5H7)

Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.

Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).

Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.

ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 5H7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Prohibitin Antibody (5H7) Summary

• Immunogen: Purified recombinant fragment of human Prohibitin expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Prohibitin Antibody (5H7)

• PHB
• PHB1
• Prohibitin

Background

Prohibitin is a 30 kD member of the band 7 family containing a SPFH domain. Prohibitin is localized the nucleus and mitochondrial inner cell membrane. This protein functions as a negative regulator of cell proliferation associated with senescence and cell death and a transcriptional repressor via recruitment of Brg-1, Brm or N-CoR, or HDAC1. Prohibitin can also function as a membrane-bound chaperone for the assembly of the mitochondrial respiratory chain complex. Prohibitin is induced by metabolic stress caused by imbalance in synthesis of mitochondrial-, nuclear-encoded mitochondrial proteins. Prohibitin can be modified by phosphorylation and ubiquitination. This protein has been shown to interact with Rb, Brg-1, Brm, N-CoR, HDAC1, and PHB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prohibitin Antibody (NBP2-37563)(1)

Publication using NBP2-37563

• Deborah Grifagni, José Malanho Silva, Leonardo Querci, Michel Lepoivre, Cindy Vallières, Ricardo O. Louro, Lucia Banci, Mario Piccioli, Marie-Pierre Golinelli-Cohen, Francesca Cantini Biochemical and cellular characterization of the CISD3 protein: Molecular bases of cluster release and destabilizing effects of nitric oxide The Journal of Biological Chemistry 2024-02-12 [PMID: 38354784]

Bioinformatics

• Gene Symbol: PHB1