Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.

Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.

Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.

Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)

Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.

Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.

Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Profilin 1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Profilin 1 Antibody (OTI1D5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Profilin 1 Antibody (OTI1D5) - Azide and BSA Free (NBP2-02577) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
• Theoretical MW: 14.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFN1