Profilin 1 Antibody (816536) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Profilin 1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Profilin 1 at approximately 17 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 816536
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Profilin 1 Antibody (816536) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Profilin 1
  Met1-Tyr140
  Accession # P07737
• Specificity: Detects human Profilin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Profilin 2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Profilin 1 Antibody (816536) [Unconjugated]

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin 1 (gene name PFN1) is a ubiquitous, cytoplasmic, 140 amino acid, 12-15 kDa actin monomer-binding protein. It facilitates adenine nucleotide exchange, supplying ATP for actin filament polymerization. It is phosphorylated and acetylated, and binds proline-rich sequences. Human Profilin 1 shares 96% amino acid sequence identity with mouse and rat Profilin 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Profilin 1 Antibody (MAB7779)(3)

Publications using MAB7779

• Ayoubi, R;McDowell, I;Fotouhi, M;Southern, K;McPherson, PS;Laflamme, C;NeuroSGC/YCharOS/EDDU collaborative group, ;ABIF Consortium, ; The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence F1000Research 2023-03-29 [PMID: 37576538] (Western Blot, Human)
• Yusuke Murakami, Mitsuhiro Nishigori, Hiroaki Yagi, Tsukasa Osaki, Masaki Wakabayashi, Manabu Shirai et al. Serum proteomic identification and validation of two novel atherosclerotic aortic aneurysm biomarkers, profilin 1 and complement factor D Proteome Science 8/5/2023 [PMID: 37543598]
• Yusuke Murakami, Mitsuhiro Nishigori, Hiroaki Yagi, Tsukasa Osaki, Masaki Wakabayashi, Manabu Shirai et al. Serum proteomic identification and validation of two novel atherosclerotic aortic aneurysm biomarkers, profilin 1 and complement factor D Proteome Science 2023-08-05 [PMID: 37543598] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PFN1
• Uniprot:
  • P07737(Human)