PRODH Antibody

Western Blot: PRODH Antibody [NBP2-27548] - (0.3 ug/ml) Analysis of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PRODH Antibody Summary

• Description: Novus Biologicals Goat PRODH Antibody (NBP2-27548) is a polyclonal antibody validated for use in WB and ELISA. Anti-PRODH Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EDQESIQPLLRHYR corresponding to internal region according to NP_057419.4, NP_001182155.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_057419.4; NP_001182155.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRODH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx. 75+55 kDa band bands observed in human brain (cerebellum and substantia nigra) lysates (calculated MW of 68.0 kDa kDa band according to NP_057419.4 and of 56.2 kDa band according to NP_001182155.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRODH Antibody

• EC 1.5.99.8
• FLJ33744
• HSPOX2
• mitochondrial
• p53-induced gene 6 protein
• PIG6MGC148078
• POX2
• PRODH2MGC148079
• proline dehydrogenase (oxidase) 1
• proline dehydrogenase (proline oxidase )
• Proline oxidase
• proline oxidase, mitochondrial
• SCZD4
• tumor protein p53 inducible protein 6

Background

The protein encoded by the PRODH gene is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. Defects in this gene are a cause of hyperprolinemia type 1 and possibly susceptibility to schizophrenia 4 (SCZD4). The gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes DiGeorge syndrome and CATCH22 syndrome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRODH Antibody (NBP2-27548)(1)

Publication using NBP2-27548

• Li T, Ma X, Hu X et al. PRODH gene is associated with executive function in schizophrenicfamilies. Am J Med Genet B Neuropsychiatr Genet 2008-07-05 [PMID: 18163391]

Bioinformatics

• Gene Symbol: PRODH
• Uniprot:
  • NP_057419.4(Human)