proBDNF Antibody

Product Discontinued

proBDNF Antibody Summary

• Immunogen: A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH
• Specificity: Detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BDNF
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Western Blot 1:1000-1:5000

Reactivity Notes

Reacts with human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water.

Alternate Names for proBDNF Antibody

• abrineurin
• brain-derived neurotrophic factor
• MGC34632
• neurotrophin

Background

Brain-derived neurotrophic factor (BDNF)is a member of the nerve growth factor family. BDNF is necessary for survival of striatal neurons in the brain and also acts as a neuroprotectant of the major neuronal types (eg. hippocampal, cortical and basal forebrain cholinergic neurons). Expression of the BDNF gene is reduced in both Alzheimer's and Huntington disease patients. Furthermore BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF protein is synthesized as a precursor, proBDNF, which is cleaved to the mature 14-kDa form. It is known that BDNF exists as a mixture of proBDNF and mature BDNF (PMID: 1265414). Although the biological activity of proBDNF is unclear, there is evidence suggesting that reductions in proBDNF levels affects neuronal degeneration in selective brain regions (eg. in Alzheimer's disease).



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for proBDNF Antibody (R-087-100)(9)

Publications using R-087-100

• B Mellstrom et al. Crit Rev Neurobiol 16, 43-9. 2004-01-01 [PMID: 15581398]
• I Tapia-Arancibia et al. Front Neuroendocrinol 25, 77-107. 2004-01-01 [PMID: 15571756]
• S Pezet et al. Brain Res Brain Res Rev 40, 240-9. 2002-01-01 [PMID: 12589922]
• XF Zhou et al. Exp. Neurol. 149: 237-42. 1998-01-01 [PMID: 9454633]
• Conner J et al. J. Neurosci. 17: 2295. 1997-01-01 [PMID: 9065491]
• XF Zhou et al. Neurosci. 74: 945-53. 1996-01-01 [PMID: 8895863]
• Q Yan et al. J. Neurosci. 14(9): 5281-91. 1994-01-01 [PMID: 8083736]
• Acheson A, Conover JC, Fandl JP, DeChiara TM, Russell M, Thadani A, Squinto SP, Yancopoulos GD, Lindsay RM. A BDNF autocrine loop in adult sensory neurons prevents cell death. Nature. 374(6521):450-3. 1995-03-30 [PMID: 7700353]
• Barde YA, Edgar D, Thoenen H. Purification of a new neurotrophic factor from mammalian brain. EMBO J. 1(5):549-53. 1982-01-01 [PMID: 7188352]

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 627(Human)
  • 12064(Mouse)
  • 24225(Rat)
• Uniprot:
  • P23560()