proBDNF Antibody

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Guinea Pig
• Conjugate: Unconjugated

proBDNF Antibody Summary

• Description: Novus Biologicals Guinea Pig proBDNF Antibody (NBP2-80497) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from mouse pro BDNF conjugated to blue carrier protein was used as the antigen.
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: BDNF
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000

Reactivity Notes

Marmoset

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole serum
• Preservative: No Preservative
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for proBDNF Antibody

• abrineurin
• brain-derived neurotrophic factor
• MGC34632
• neurotrophin

Background

Brain-derived neurotrophic factor (BDNF)is a member of the nerve growth factor family. BDNF is necessary for survival of striatal neurons in the brain and also acts as a neuroprotectant of the major neuronal types (eg. hippocampal, cortical and basal forebrain cholinergic neurons). Expression of the BDNF gene is reduced in both Alzheimer's and Huntington disease patients. Furthermore BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF protein is synthesized as a precursor, proBDNF, which is cleaved to the mature 14-kDa form. It is known that BDNF exists as a mixture of proBDNF and mature BDNF (PMID: 1265414). Although the biological activity of proBDNF is unclear, there is evidence suggesting that reductions in proBDNF levels affects neuronal degeneration in selective brain regions (eg. in Alzheimer's disease).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BDNF