proBDNF Antibody

Western Blot: proBDNF Antibody [NBP2-44063] - Detection of recombinant human BDNF raised in E.coli.

Product Discontinued

proBDNF Antibody Summary

• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human proBDNF protein produced in E.coli.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BDNF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:1000
• Application Notes: This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.

Reactivity Notes

Cross reactivity with proBDNF from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: IgG purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water.

Alternate Names for proBDNF Antibody

• abrineurin
• brain-derived neurotrophic factor
• MGC34632
• neurotrophin

Background

Brain-derived neurotrophic factor (BDNF)is a member of the nerve growth factor family. BDNF is necessary for survival of striatal neurons in the brain and also acts as a neuroprotectant of the major neuronal types (eg. hippocampal, cortical and basal forebrain cholinergic neurons). Expression of the BDNF gene is reduced in both Alzheimer's and Huntington disease patients. Furthermore BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF protein is synthesized as a precursor, proBDNF, which is cleaved to the mature 14-kDa form. It is known that BDNF exists as a mixture of proBDNF and mature BDNF (PMID: 1265414). Although the biological activity of proBDNF is unclear, there is evidence suggesting that reductions in proBDNF levels affects neuronal degeneration in selective brain regions (eg. in Alzheimer's disease).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BDNF