PRMT8 Antibody - Azide and BSA Free

Western Blot: PRMT8 Antibody [NBP3-15568] - analysis of extracts from normal (control) and PRMT8 Rabbit pAb knockout (KO) 293T cells, using PRMT8 Rabbit pAb antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.

Product Discontinued

PRMT8 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human PRMT8 (NP_062828.3). VIFARDKWLKPGGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDIYTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRRGEEIYGTISMKPNAKNVRDLDFTVDLDFKG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for PRMT8 Antibody - Azide and BSA Free

• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.77
• Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
• HMT1 hnRNP methyltransferase-like 3 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 3
• HMT1 hnRNP methyltransferase-like 4 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 4
• HRMT1L3
• HRMT1L4
• PRMT8
• protein arginine methyltransferase 8
• protein arginine N-methyltransferase 4
• protein arginine N-methyltransferase 8

Background

PRMT8 codes for a protein with a length of 394 amino acids, weighing approximately 45 kDa, and has a shorter isoform with a length of 385 amino acids and a weight of approximately 44 kDa. This gene codes for a membrane-associated argining methyltransferase that helps mediate the formation of MMA and aDMA as well as mono- and dimethylate the EWS protein. Current studies are being done on several diseases and disorders relating to this gene including coronary heart disease, ataxia, sarcoma, and malaria. PRMT8 has also been shown to have interactions with COIL, HNRNPA1, SYNCRIP, and PRMT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: PRMT8