PRMT6 Antibody

Western Blot: PRMT6 Antibody [NB100-56642] - Mouse 4T1 cells and EMT6 cells whole cell lysate. Image from verified customer review.

Western Blot: PRMT6 Antibody [NB100-56642] - Analysis of PRMT6 in HeLa lysate using PRMT6 antibody at 1:500 dilution.

• Reactivity: Hu, Mu
• Applications: WB, Func
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRMT6 Antibody Summary

• Description: Novus Biologicals Rabbit PRMT6 Antibody (NB100-56642) is a polyclonal antibody validated for use in WB. Anti-PRMT6 Antibody: Cited in 20 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed a synthetic peptide corresponding to amino acids 23-43 (CEEDGAEREAALERPRRTKRER) of human PRMT6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • In vitro assay reported in scientific literature (PMID 29973649)
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for PRMT6 Antibody

• EC 2.1.1.-
• EC 2.1.1.125
• FLJ10559
• FLJ51477
• Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
• Histone-arginine N-methyltransferase PRMT6
• HMT1 hnRNP methyltransferase-like 6 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 6
• HRMT1L6
• PRMT6
• protein arginine methyltransferase 6
• protein arginine N-methyltransferase 6

Background

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT6 was identified by searching the human genome for protein arginine N-methyltransferase (PRMT) family members. PRMT6 cDNA codes for a protein of approximate molecular weight of 42 kDa consisting of a catalytic core sequence common to other PRMT enzymes. PRMT6 demonstrates type I PRMT activity, capable of forming both >NG-monomethylarginine and asymmetric >NG,NG-dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. A comparison of substrate specificity reveals that PRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition to methylating substrate proteins, PRMT6 displays automethylation activit. GFP-fusion protein expression in the transfected cell lines suggest that PRMT6 localizes in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT6 Antibody (NB100-56642)(21)

Publications using NB100-56642

• Khalil M, Ismail H, Panasyuk G et al. Asymmetric Dimethylation of Ribosomal S6 Kinase 2 Regulates Its Cellular Localisation and Pro-Survival Function International journal of molecular sciences 2023-05-15 [PMID: 37240151]
• Wang Z, Pan Z, Adhikari S, et al. m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region The EMBO journal 2021-01-18 [PMID: 33459381]
• Zhang H, Han C, Li T et al. The methyltransferase PRMT6 attenuates antiviral innate immunity by blocking TBK1-IRF3 signaling. Cell. Mol. Immunol. 2018-07-04 [PMID: 29973649]
• Di Lorenzo A. Gain-of-function Mouse Models to Investigate Biological Roles of PRMT6. Thesis 2014-01-01 (WB, Human)
• Kim JK, Lim Y, Lee JO et al. PRMT4 is involved in insulin secretion via methylation of histone H3 in pancreatic beta-cells J. Mol. Endocrinol. 2015-04-27 [PMID: 25917831] (WB, Human)
  
  Details:
  PRMT6 antibody was used for WB analysis of lysates from pancreatic beta-cell line/HIT-T15 b cells which was pre-incubated in KRBH buffer without glucose for 1 h and then stimulated for different time intervals with 16.7 mM glucose in KRBH buffer (Figure 1B).
• Lorenzo AD, Yang Y, Macaluso M, Bedford MT. Gain-of-function mouse models to investigated biological roles of PRMT6. Nucleic Acids Res. 2014-06-17 [PMID: 24939901] (WB, Mouse, Human)
  
  Details:
  Fig 16B, human-PRMT6 transgenic &WT mouse embryo fibroblasts. The specificity of the PRMT6 was validated in PRMT6 transgenic cells by WB (Fig 16B).
• Obianyo O, Causey CP, Jones JE, Thompson PR. Activity-based protein profiling of protein arginine methyltransferase 1. ACS Chem Biol. 2011-10-21 [PMID: 21838253] (WB)
  
  Details:
  WB (MCF-7), Fig 4B.
• Lim Y, Hong E, Kwon D et al. Proteomic identification and comparative analysis of asymmetrically arginine-methylated proteins in immortalized, young and senescent cells. Electrophoresis. 2010-12-01 [PMID: 21080485] (WB)
  
  Details:
  WB: (immortalized WI-38 VA13 fibroblasts, normal young WI-38 fibroblasts, fully senescent WI-38 fibroblasts), Fig 1A, B. Note: Fully senescent cells were obtained by routinely subculturing young fibroblasts and then maintaining the replicatively growth-ar
• Kim C, Lim Y, Yoo BC et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta. 2010-09-01 [PMID: 20541591] (WB)
  
  Details:
  WB (HeLa), Fig 2A
• Yu Z, Chen T, Hebert J et al. A mouse PRMT1 null allele defines an essential role for arginine methylation in genome maintenance and cell proliferation. Mol Cell Biol. 2009-06-01 [PMID: 19289494] (WB, Mouse)
  
  Details:
  WB (mouse embryonic fibroblasts), Fig. 2E.
• Herrmann F, Pably P, Eckerich C et al. Human protein arginine methyltransferases in vivo--distinct properties of eight canonical members of the PRMT family. J Cell Sci. 2009-03-01 [PMID: 19208762] (WB)
  
  Details:
  1. IMG-496 (PRMT4): WB (HEk 293 cells), Fig. 1C,B. 2. IMG-506 (PRMT6): WB (HEk 293 cells), Fig. 1C,B.
• Lim Y, Lee E, Lee J et al. Down-regulation of asymmetric arginine methylation during replicative and H2O2-induced premature senescence in WI-38 human diploid fibroblasts. J Biochem. 2008-10-01 [PMID: 18676353] (WB, Human)
  
  Details:
  WB (WI-38 normal human diploid fibroblasts), Fig. 1D, 2D.
• Fronz K, Otto S, Kolbel K et al. Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior. J Biol Chem. 2008-07-18 [PMID: 18495660] (WB)
  
  Details:
  WB(HeLa cells), Fig. 5.
• Kitowska K, Zakrzewicz D, K0nigshoff M et al. Functional role and species-specific contribution of arginases in pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2008-01-01 [PMID: 17934065]
• Guccione E, Bassi C, Casadio F et al. Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive. Nature. 2007-10-18 [PMID: 17898714] (WB)
  
  Details:
  WB [Fig. 2a (PRMT6 SiRNA transfected HeLa cells), 2b (PRMT6 transfected HeLa cells), Sup Fig. 6 (PRMT6 SiRNA transfected HeLa cells)]. The antibody was SiRNA validated in Fig. 2a and PRMT6-transfected validated by western blot in Fig. 2b. Endogenous level
• Xie B, Invernizzi CF, Richard S, Wainberg MA. Arginine methylation of the human immunodeficiency virus type 1 Tat protein by PRMT6 negatively affects Tat Interactions with both cyclin T1 and the Tat transactivation region. J Virol. 2007-04-01 [PMID: 17267505] (WB)
  
  Details:
  WB (Fig 5), HeLa, Jurkat, and 293T cells), SiRNA validated (Fig 5, in HeLa, Jurkat, and 293T cells).
• Bulau P, Zakrzewicz D, Kitowska K et al. Analysis of methylarginine metabolism in the cardiovascular system identifies the lung as a major source of ADMA. Am J Physiol Lung Cell Mol Physiol. 2007-01-01 [PMID: 16891395]
• Herrmann F, Lee J, Bedford MT et al. Dynamics of human protein arginine methyltransferase 1(PRMT1) in vivo. J Biol Chem. 2005-11-11 [PMID: 16159886]
• Lim Y, Kwon YH, Won NH et al. Multimerization of expressed protein-arginine methyltransferases during the growth and differentiation of rat liver. Biochim Biophys Acta. 2005-05-25 [PMID: 15837430]
• Boulanger MC, Liang C, Russell RS et al. Methylation of Tat by PRMT6 regulates human immunodeficiency virus type 1 gene expression. J Virol. 2005-01-01 [PMID: 15596808]
• Herrmann F, Bossert M, Schwander A et al. Arginine methylation of scaffold attachment factor A by heterogeneous nuclear ribonucleoprotein particle-associated PRMT1. J Biol Chem. 2004-11-19 [PMID: 15364944]

Reviews for PRMT6 Antibody (NB100-56642) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: BT-20 human breast cancer cell line,SUM-149
• Species: Human
• Lot: 111302B-01

reviewed by: Verified Customer WB Human 12/27/2019

Summary

• Application: Western Blot
• Sample Tested: bt549
• Species: Human
• Lot: 111302B-01

reviewed by: Verified Customer WB Mouse 10/17/2018

Summary

• Application: Western Blot
• Sample Tested: 4T1 cells whole cell lysate,EMT6 cells
• Species: Mouse
• Lot: 111302B-01

Bioinformatics

• Gene Symbol: PRMT6
• Entrez:
  • 55170(Human)
  • 99890(Mouse)
• Uniprot:
  • Q96LA8(Human)