PRMT6 Antibody

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Western Blot: PRMT6 Antibody [NB100-56642] - Analysis of PRMT6 in HeLa lysate using PRMT6 antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PRMT6 Antibody Summary

Immunogen
This antibody was developed a synthetic peptide corresponding to amino acids 23-43 (CEEDGAEREAALERPRRTKRER) of human PRMT6.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRMT6
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Publications
Read Publications using
NB100-56642 in the following applications:

  • WB
    12 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PRMT6 Antibody

  • EC 2.1.1.-
  • EC 2.1.1.125
  • FLJ10559
  • FLJ51477
  • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
  • Histone-arginine N-methyltransferase PRMT6
  • HMT1 hnRNP methyltransferase-like 6 (S. cerevisiae)
  • HMT1 hnRNP methyltransferase-like 6
  • HRMT1L6
  • protein arginine methyltransferase 6
  • protein arginine N-methyltransferase 6

Background

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT6 was identified by searching the human genome for protein arginine N-methyltransferase (PRMT) family members. PRMT6 cDNA codes for a protein of approximate molecular weight of 42 kDa consisting of a catalytic core sequence common to other PRMT enzymes. PRMT6 demonstrates type I PRMT activity, capable of forming both >NG-monomethylarginine and asymmetric >NG,NG-dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. A comparison of substrate specificity reveals that PRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition to methylating substrate proteins, PRMT6 displays automethylation activit. GFP-fusion protein expression in the transfected cell lines suggest that PRMT6 localizes in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRMT6 Antibody (NB100-56642)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(12)
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Human
(4)
Mouse
(2)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-56642 Applications Species
Kim JK, Lim Y, Lee JO et al. PRMT4 is involved in insulin secretion via methylation of histone H3 in pancreatic beta-cells J. Mol. Endocrinol. 2015 Apr 27 [PMID: 25917831] (WB, Human)

Details:
PRMT6 antibody was used for WB analysis of lysates from pancreatic beta-cell line/HIT-T15 b cells which was pre-incubated in KRBH buffer without glucose for 1 h and then stimulated for different time intervals with 16.7 mM glucose in KRBH buffer (Figure 1B).
WB Human
Lorenzo AD, Yang Y, Macaluso M, Bedford MT. Gain-of-function mouse models to investigated biological roles of PRMT6. Nucleic Acids Res. 2014 Jun 17 [PMID: 24939901] (WB, Human, Mouse)

Details:
Fig 16B, human-PRMT6 transgenic &WT mouse embryo fibroblasts. The specificity of the PRMT6 was validated in PRMT6 transgenic cells by WB (Fig 16B).
WB Human, Mouse
Obianyo O, Causey CP, Jones JE, Thompson PR. Activity-based protein profiling of protein arginine methyltransferase 1. ACS Chem Biol. 2011 Oct 21 [PMID: 21838253] (WB)

Details:
WB (MCF-7), Fig 4B.
WB
Lim Y, Hong E, Kwon D et al. Proteomic identification and comparative analysis of asymmetrically arginine-methylated proteins in immortalized, young and senescent cells. Electrophoresis. 2010 Dec [PMID: 21080485] (WB)

Details:
WB: (immortalized WI-38 VA13 fibroblasts, normal young WI-38 fibroblasts, fully senescent WI-38 fibroblasts), Fig 1A, B. Note: Fully senescent cells were obtained by routinely subculturing young fibroblasts and then maintaining the replicatively growth-ar
WB
Kim C, Lim Y, Yoo BC et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta. 2010 Sep [PMID: 20541591] (WB)

Details:
WB (HeLa), Fig 2A
WB
Yu Z, Chen T, Hebert J et al. A mouse PRMT1 null allele defines an essential role for arginine methylation in genome maintenance and cell proliferation. Mol Cell Biol. 2009 Jun [PMID: 19289494] (WB, Mouse)

Details:
WB (mouse embryonic fibroblasts), Fig. 2E.
WB Mouse
Herrmann F, Pably P, Eckerich C et al. Human protein arginine methyltransferases in vivo--distinct properties of eight canonical members of the PRMT family. J Cell Sci. 2009 Mar 1 [PMID: 19208762] (WB)

Details:
1. IMG-496 (PRMT4): WB (HEk 293 cells), Fig. 1C,B. 2. IMG-506 (PRMT6): WB (HEk 293 cells), Fig. 1C,B.
WB
Lim Y, Lee E, Lee J et al. Down-regulation of asymmetric arginine methylation during replicative and H2O2-induced premature senescence in WI-38 human diploid fibroblasts. J Biochem. 2008 Oct [PMID: 18676353] (WB, Human)

Details:
WB (WI-38 normal human diploid fibroblasts), Fig. 1D, 2D.
WB Human
Fronz K, Otto S, Kolbel K et al. Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior. J Biol Chem. 2008 Jul 18 [PMID: 18495660] (WB)

Details:
WB(HeLa cells), Fig. 5.
WB
Kitowska K, Zakrzewicz D, K0nigshoff M et al. Functional role and species-specific contribution of arginases in pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2008 Jan [PMID: 17934065]
Show All 18 Publications.

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Blogs on PRMT6.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

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Bioinformatics

Gene Symbol PRMT6
Entrez
Uniprot