PRL-3/PTP4A3 Antibody (334402)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL‑3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL‑3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 334402
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PRL-3/PTP4A3 Antibody (334402) Summary

• Immunogen: E. coli-derived recombinant human PRL-3
  Ala2-Met173
  Accession # O75365
• Specificity: Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTP4A3
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRL-3/PTP4A3 Antibody (334402)

• PRL3
• PRL-3
• PRL3EC 3.1.3.48
• PRL-3potentially prenylated protein tyrosine phosphatase
• PRL-Rprotein tyrosine phosphatase type IVA 3
• protein tyrosine phosphatase type IVA, member 3
• Protein-tyrosine phosphatase 4a3
• Protein-tyrosine phosphatase of regenerating liver 3
• PTP4A3

Background

Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRL-3/PTP4A3 Antibody (MAB3219)(4)

Publications using MAB3219

• Trenkmann M, Brock M, Gay R, Michel B, Gay S, Huber L Tumor necrosis factor alpha-induced microRNA-18a activates rheumatoid arthritis synovial fibroblasts through a feedback loop in NF-kappaB signaling. Arthritis Rheum, 2013;65(4):916-27. 2013 [PMID: 23280137] (Western Blot, Human)
• Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma. Int. J. Cancer, 2010;127(3):543-54. 2010 [PMID: 19960436] (Western Blot, Human)
• Wang Z, He YL, Cai SR, Zhan WH, Li ZR, Zhu BH, Chen CQ, Ma JP, Chen ZX, Li W, Zhang LJ Expression and prognostic impact of PRL-3 in lymph node metastasis of gastric cancer: its molecular mechanism was investigated using artificial microRNA interference. Int. J. Cancer, 2008;123(6):1439-47. 2008 [PMID: 18561324] (IHC-P, Human)
• Daouti S, Li WH, Qian H, Huang KS, Holmgren J, Levin W, Reik L, McGady DL, Gillespie P, Perrotta A, Bian H, Reidhaar-Olson JF, Bliss SA, Olivier AR, Sergi JA, Fry D, Danho W, Ritland S, Fotouhi N, Heimbrook D, Niu H A selective phosphatase of regenerating liver phosphatase inhibitor suppresses tumor cell anchorage-independent growth by a novel mechanism involving p130Cas cleavage. Cancer Res., 2008;68(4):1162-9. 2008 [PMID: 18281492] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PTP4A3
• Uniprot:
  • O75365()