PRKCBP1 Antibody

Western Blot: PRKCBP1 Antibody [NBP3-18649] - Figure 2. Western blot analysis using the antibody directed against PRKCBP1 Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against PRKCBP1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.

ELISA: PRKCBP1 Antibody [NBP3-18649] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PRKCBP1 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRKCBP1 Antibody Summary

• Description: Novus Biologicals Rabbit PRKCBP1 Antibody (NBP3-18649) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against human PRKCBP1 (zinc finger, MYND-type containing 8), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZMYND8
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:500
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Knockdown Validated
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for PRKCBP1 Antibody

• ESP-2
• HED-2
• zyxin
• zyxin-2

Background

PRKCBP1 is encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZMYND8
• Uniprot:
  • Q9ULU4(Human)